Benzoic acid, compound with cyclohexylamine (1:1)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 December 2002 to 13 December 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Reliable experimental study following OECD guidelines (not specified), ISO14669:1999 (E) and GLP standards.

Qualifier:

according to guideline

Guideline:

other: ISO14669:

Version / remarks:

1999 (E)

Deviations:

not specified

Principles of method if other than guideline:

ISO14669:1999 (E) - Water Quality - Determination of Acute Lethal Toxicity to Marine Copepods (Copepoda, Crustacea).

GLP compliance:

yes

Specific details on test material used for the study:

The test substance is yclohexylamine benzoate. The test substance was obtained from the study Sponsor on 5th September 2002 and stored in the original container at 15 ± 2°C.

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Acartia tonsa – Marine copepod

Details on test organisms:

The test was carried out using Acartia tonsa (12 -15 days old) obtained from Guernsey Sea Farms, Guernsey.

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.0 °C to 20.0 °C

pH:

7.22 to 8.11

Dissolved oxygen:

84.7 %ASV to 97.0 %ASV

Nominal and measured concentrations:

An experimental study was conducted with test concentrations of 20.0, 35.6, 63.2, 112.4, 200.0 and 355.6 mg/L.

Reference substance (positive control):

yes

Remarks:

3, 5- dicholorphenol

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

136.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 355.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Raw results with reference substance 3,5-dichlorophenol were reported, but the EC50 was not calculated.

Validity criteria fulfilled:

not specified

Conclusions:

The test substance did induce acute immobilisation of Acartia tonsa at the nominal test concentrations after 48 h of exposure. The 24- and 48-hour EC50 values were reported as >355.6 mg/L and 136.6 mg/L, respectively

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the marine copepod Acartia tonsa following the guideline ISO 14669:1999 (E). Acute immobilisation of Acartia tonsa was observed; the 48-h EC₅₀ was reported as = 136.6 mg/L (nominal). 

Key value for chemical safety assessment

Marine water invertebrates

Marine water invertebrates

Effect concentration:

136.6 mg/L