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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 December 2002 to 13 December 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Justification for type of information:
Reliable experimental study following OECD guidelines (not specified), ISO14669:1999 (E) and GLP standards.
Qualifier:
according to
Guideline:
other: ISO14669:
Version / remarks:
1999 (E)
Deviations:
not specified
Principles of method if other than guideline:
ISO14669:1999 (E) - Water Quality - Determination of Acute Lethal Toxicity to Marine Copepods (Copepoda, Crustacea).
GLP compliance:
yes
Specific details on test material used for the study:
The test substance is yclohexylamine benzoate. The test substance was obtained from the study Sponsor on 5th September 2002 and stored in the original container at 15 ± 2°C.
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Acartia tonsa – Marine copepod
Details on test organisms:
The test was carried out using Acartia tonsa (12 -15 days old) obtained from Guernsey Sea Farms, Guernsey.
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.0 °C to 20.0 °C
pH:
7.22 to 8.11
Dissolved oxygen:
84.7 %ASV to 97.0 %ASV
Nominal and measured concentrations:
An experimental study was conducted with test concentrations of 20.0, 35.6, 63.2, 112.4, 200.0 and 355.6 mg/L.
Reference substance (positive control):
yes
Remarks:
3, 5- dicholorphenol
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
136.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 355.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
Raw results with reference substance 3,5-dichlorophenol were reported, but the EC50 was not calculated.
Validity criteria fulfilled:
not specified
Conclusions:
The test substance did induce acute immobilisation of Acartia tonsa at the nominal test concentrations after 48 h of exposure. The 24- and 48-hour EC50 values were reported as >355.6 mg/L and 136.6 mg/L, respectively

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the marine copepod Acartia tonsa following the guideline ISO 14669:1999 (E). Acute immobilisation of Acartia tonsa was observed; the 48-h EC50 was reported as = 136.6 mg/L (nominal). 

Key value for chemical safety assessment

EC50/LC50 for marine water invertebrates:
136.6 mg/L

Additional information