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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
9 December 2002 to 12 December 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Reliable experimental study following ISO/DIS 10253 and GLP standards.
Qualifier:
according to
Guideline:
ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
Version / remarks:
Not Specified
Deviations:
not specified
GLP compliance:
yes
Specific details on test material used for the study:
The test substance is cyclohexylamine benzoate. The test substance was obtained from the study Sponsor on 5th September 2002 and stored in the original container at 15 ± 2°C.
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Skeletonema costatum
Details on test organisms:
The test organism, Skeletonema costatum CCAP1077/5, was obtained from Culture Collection of Algae and Protozoa,Dunstaffnage.
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
18 °C at 0 h and 19.5 to 20.5 °C during the test
pH:
7.00 to 8.31
Dissolved oxygen:
90.5% - 99.8 % Air Saturation Values
Nominal and measured concentrations:
An experimental study was conducted with nominal test concentrations of 10.0, 17.8, 31.6, 56.2, and 100 mg/L.
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol was used as the positive reference control substance.
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
17.84 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other:
Remarks:
12.26 -25.40 mg/L 95% Fiducial Limits
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
37.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other:
Remarks:
25.99 -188.04 mg/L Fiducial Limits
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
17.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
Raw results with reference substance 3,5-dichlorophenol were reported, but the EC50 was not calculated.
Validity criteria fulfilled:
not specified
Conclusions:
The test substance caused a reduction of growth in the algal cultures (test units) exposed to a series of nominal test concentrations of the test item; therefore, there were toxic or inhibitory effects. The 72-h EC50 (based on growth rate) was calculated to be 17.84 mg/L. The 72-h NOEC (based on growth rate) was calculated to be 10 mg/L).

Description of key information

The key study was a static acute toxicity test on the effects of the substance on the marine alga, Skeletonema costatum, following guideline ISO/DIS 10253The test substance caused a reduction of growth in the algal cultures (test units) exposed to a series of nominal test concentrations of the test item. The 72-h EC50 (based on growth rate) was calculated to be 17.84 mg/L. The 72-h NOEC and 72-h LOEC (each based on growth rate) were reported as 10.0 mg/L and 17.8 mg/L, respectively.

Key value for chemical safety assessment

EC50 for marine water algae:
17.84 mg/L
EC10 or NOEC for marine water algae:
10 mg/L

Additional information