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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 November 2002 to 29 November 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Deviations:
not specified
GLP compliance:
yes
Specific details on test material used for the study:
The test substance is cyclohexylamine benzoate. The test substance was obtained from the study Sponsor on 5th September 2002 and stored in the original container at 15 ± 2°C.
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Scophthalmus maximus (Marine turbot).
Details on test organisms:
The test organisms were juvenile Scophthalmus maximus (turbot). The organisms were obtained from a supplier, France Turbot, L‛ Epine France on 2 October 2002. The fish were free of malformations and in good health prior to the definitive test.
Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not reported
Test temperature:
The temperature of the test medium was 13.5 -15.0°C.
pH:
The pH values throughout the test were 7.23 – 8.77.
Dissolved oxygen:
The dissolved oxygen was 81.1 % - 98.9% air saturation values.
Salinity:
The salinity was 33.5 - 35.2 parts per thousand.
Nominal and measured concentrations:
The study was conducted with nominal test concentrations of 100.0, 177.8, 316.2, 562.3, and 1000.0 mg/L.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
All animals in the control vessels survived the 96-hour test period. After 96 hours exposure to concentrations of the test substance, the LC50 value was determined to be >1000.0 mg/L. The 24-h, 48-h and 72-h values were also determined to be >1000.0 mg/L.
Validity criteria fulfilled:
not specified
Conclusions:
Exposure of Scophthalmus maximus to a series of test concentrations showed no mortality. The 96-h LC50 and the 96-h NOEC were each reported as 1000 mg/L.

Description of key information

The key study was a semi-static acute toxicity test on the effects of the substance on the marine turbot fish, Scophthalmus maximus following OECD guideline 203. Exposure of Scophthalmus maximus to a series of test concentrations showed no mortality. The 96-h LC50 and 96 -h NOEC were each reported as > 1000 mg/L. .

Key value for chemical safety assessment

LC50 for marine water fish:
1 000 mg/L

Additional information