6,10-dimethylundeca-5,9-dien-2-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non GLP, Guideline study according to national standard protocol. No analytical monitoring.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, Part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundeca-5,9-dien-2-one

EC Number:

211-711-2

EC Name:

6,10-dimethylundeca-5,9-dien-2-one

Cas Number:

689-67-8

Molecular formula:

C13H22O

IUPAC Name:

6,10-dimethylundeca-5,9-dien-2-one

Details on test material:

- Name of test material (as cited in study report): Geranylacetone
Test substance No.: 88/604
- Analytical purity: 92 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of the test item: The test item was dissolved in acetone (1 g/100 ml) and was added to the test water, subsequently the fish were placed into the aquaria. Similarily to the highest amount of solvent used, 1 ml acetone/l was added to the control aquarium.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.0 cm (range 5.5 - 7.1 cm)
- Weight at study initiation (mean and range, SD): 1.8 g (range 1.2 - 2.8 g)
- Feeding during test. 1 day before and during exposure
- Housing: Duration of housing: about 1 month Mortality during the last 2 weeks of housing: 1.4 % , Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride.

ACCLIMATION
- Acclimation period: 3 days
- Health during acclimation (any mortality observed):no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

23 °C

pH:

7.4 - 7.8

Dissolved oxygen:

7.1 - 8.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 1.0, 2.15, 4.64 and 10.0 mg/l. In addition, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 1.8 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Ratio Na/K ions: 10 : 1
- Acid capacity: 0.8 mmol/l
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: pH and oxygen content after 0, 24, 48, 72 and 96 h


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6.78 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

96 h NOEC 2.2 mg/L (nominal) based on test material
96 h LC0 4.64 mg/L (nominal) based on test material
96 h LC100 10 mg/L (nominal) based on test material

Results with reference substance (positive control):

The LC50 (48 h) of the positive control chloroacetamide was 23 mg/l, which corresponds to the normal sensitivity of the fish.

Reported statistics and error estimates:

The LC50 was statistically evaluated as the geometric mean of the LC0 (4.64 mg/l) and the LC100 (10 mg/l) nominal values.