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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 48h Daphnia magna > 100 mg/l

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

No studies on the acute toxicity to aquatic invertebrates were performed on the substance under registration (CAS 13863-31-5). Therefore, the available data on similar substances belonging to the category of Stilbene Fluorescent Whitening Agents, group 3, have been taken into account.


The tested substances CAS 4193-55-9, CAS 70942-01-7 and CAS 16470-24-9 are the dihydroxyethylamino derivatives disulphonated sodium salt, the dihydroxyethylamino derivatives sodium/potassium salt and the dihydroxyethylamino derivatives tetrasulphonated, respectively. In the case of the CAS 70942-01-7 the presence of potassium ion has no relevant toxicological influence for the endpoint.

For all the analogous water solubility is higher than for the substance under registration (CAS 13863-31-5), therefore they are more bioavailable in the water compartment and can be considered as conservative examples.

The chemical structures are very similar, with Tanimoto similarity higher than 95 %: the substance under registration has a methyl group where the analogues has a hydroxyethyl substituent on the nitrogen atom. Based on the OECD Toolbox modelling of the liver metabolisation, both structures share the same monohyroxy derivative as main metabolite. Biological reactivity for the substance under registration is therefore not expected higher that the considered analogues substances.

For all the tested analogous a value of EC50 greater than 100 mg/l was set, thus for the substance under registration the limit value of 100 mg/l has been taken as representative EC50.