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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Description of key information

No bioaccumulation is expected

Key value for chemical safety assessment

BCF (aquatic species):
0.5 dimensionless

Additional information

According to the REACH Regulation, Annex IX, Column 2, the substance does not need to be tested due to the low potential for bioaccumulation (log Kow < 3) and a low potential to cross biological membranes (high molecular weight, high polarity); furthermore direct and indirect exposure of the aquatic compartment is unsuitable.

Several bioaccumulation studies are available on the substance demonstrating no bioaccumulation in fish.

Bluegills (Lepomis machrochirus) have been exposed in dynamic flowthrough tank-water systems to test item to determine to what degree this product accumulate in portions of the fish which may be consumed by humans. Test concentration was 0.1 mg/l. The exposure period was 35 days and the elimination phase was 7 days. During the tests, fish and water were periodically sampled and analysed. Furthermore an accumulation study using 14C-labeled substance was performed at concentrations of 0.01 and 0.001 mg/l; exposure phase was of 30 days, while elimination phase of 14 days. Only sporadic trace concentrations (less than 0.05 mg/kg) of the test item were present in the fish at exposure levels of 1 mg/l. In most instances, no detectable substance was found. The data indicates that there is no tendency for the substance to be taken up and stored in the fish. In the case of the 14C-substance, at both of the concentrations tested the levels of radioactivity found in the fish were approximately equal to those in the tank-water throughout the study. Furthermore the absence of accumulated radioactivity demonstrated that neither parent compound nor possible metabolites of the substance are accumulated in fish (Ganz, 1975).

The test substance was also tested to determine its potential for significant accumulation in the flesh of the Bluegill (Lepomis macrochirus) and channel catfish (Ictalurus punctatus). The test was conducted at the following concentrations: 50 µg/l, 5 µg/l, and 0.5 µg/l total test substances (the study was conducted with five agents administrated in the same solutions). The results of the bluegill analysesexposedto a nominal concentration of 50 µg/l total agents (12.5 µg/l of each one) indicate that no accumulation of the substance of the interest was observed at measurable levels (i.e., greater than 10 µg/kg). At the concentration of 5 and 0.5 µg/l(1.25 and 0.125 µg/l, respectively) no measurable accumulation was detected (Sturm, 1974).

In the Bionomics Inc. and Ciba Geigy Ltd. (1972-1973) study sixty Bluegill were continuously exposed to the chemical in water for a period of at least 28 days. After this period, the fish will be transferred for a period of 7 to uncontaminated water and after this period they were sampled. Generally the test item concentrations were not determinable in tissues or were too low to be quantified. In one case, a value of 0.03 mg/kg close to the sensitivity limit (0.01 mg/kg) was measured, confirming that the bioaccumulation potential of the test substance is considered negligible.

For completeness sake, the estimation of biodegradation using the BCFBAF v3.01[1] tool was performed.

The BCF estimated value from regression-based method is 0.500 (BCF = 3.162 l/kg wet-wt), in agreement with the common value of non bioaccumulable substances.

[1]BCFBAF v.3.01 is an application contained in the EpiSuite 4.1, the suite of physical/chemical property and environmental fate estimation programs developed by the EPA’s Office of Pollution Prevention Toxics and Syracuse Research Corporation (SRC).