Registration Dossier

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity 99.8 %
AZ 04803 of September 5th, 1990, Analytisches Laboratorium Hoechst AG

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: HOECHST AG, Kastengrund, SPF-Zucht
- Strain: Hoe: WISKf (SPF71)
- Age at study initiation: 8 - 10 weeks
- Weight at study initiation: males: 186 g - 206 g (average 198 g); females: 183 g - 202 g (average 193 g)
- Housing: 5 animals per makrolon cage
- Diet: Altromin 1324 rat diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 50 +/- 20%
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:
inhalation: aerosol
Type of inhalation exposure:
nose only
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Gas chromatography with external standard
Duration of exposure:
4 h
Concentrations:
1.74, 3.0, 7.3 mg/L
No. of animals per sex per dose:
5 animals per sex per dose in the treatment groups
Details on study design:
- Duration of observation period following administration: all animals 14 days and group with highest exposure 21 days
- Frequency of observations: exact observation during inhalation period
- Frequency of weighing: before exposure, day 7, 14 and 21 post exposure
- Necropsy of survivors performed: yes

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 7.3 mg/L air (analytical)
Exp. duration:
4 h
Mortality:
no animals died during exposure or post exposure period

Applicant's summary and conclusion

Conclusions:
Based on the results of this study the 4h LC50 of 2-chlorobenzal chloride is greater than 7.3 mg/L.