L-methionine

Developmental toxicity / teratogenicity

Administrative data

Endpoint:

developmental toxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Untersuchungen zur Auswirkung von Aminosaurenuberdosierungen auf die Foetalphase bei Ratten.

Author:

Kaemmerer, K. and Aly, Z.H.

Year:

1975

Bibliographic source:

DTW Dtsch Tierarztl Wochenschr; 82 (11) p. 457-460.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Pregnant rats were given high doses of essential amino acids as food supplement during the so-called critical phase from the 10th to the 20th day of pregnancy.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

L-methionine

EC Number:

200-562-9

EC Name:

L-methionine

Cas Number:

63-68-3

Molecular formula:

C5H11NO2S

IUPAC Name:

L-methionine

Details on test material:

- Name of test material (as cited in study report): L-Methionin

Test animals

Species:

rat

Strain:

Wistar

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Age at study initiation:no data
- Weight at study initiation: when mated female rats had a weight between 170 to 190 g
- Fasting period before study:no data
- Housing: individually
- Diet (e.g. ad libitum):no data
- Water (e.g. ad libitum):no data
- Acclimation period:no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C):22-25°C
- Humidity (%): 60%
- Air changes (per hr):no data
- Photoperiod (hrs dark / hrs light):no data

Administration / exposure

Route of administration:

oral: feed

Details on exposure:

Animals received basal diet from day 1 to day 9 post coitum. From day 10 to day 20 post coitum animals received basal diet supplemented with L-methionine. From day 20 to day 22 animals received again basal diet.

Analytical verification of doses or concentrations:

not specified

Details on mating procedure:

Mating was carried out by placing three females into the cage of one male over night and confirmed by the presence of a vaginal plug.

Duration of treatment / exposure:

Animals received L-methionine supplemented feed from day 10 to day 20 post coitum

Control animals:

yes, plain diet

Examinations

Maternal examinations:

weigth gain

Ovaries and uterine content:

The ovaries and uterine content was examined after termination: Yes
Examinations included:
- Gravid uterus weight: No data
- Number of corpora lutea: Yes
- Number of implantations: Yes
- Number of resorptions: Yes

Fetal examinations:

- External examinations: Yes
- Soft tissue examinations: Yes
- Skeletal examinations: Yes
- Head examinations: Yes

Results and discussion

Results: maternal animals

Maternal developmental toxicity

Details on maternal toxic effects:

Maternal toxic effects:yes

Details on maternal toxic effects:
food supplemented with L-methionine resulted a reduced food consumption and a reduced weight gain

Results (fetuses)

Details on embryotoxic / teratogenic effects:

Embryotoxic / teratogenic effects:yes

Details on embryotoxic / teratogenic effects:
large doses of L-methionine resulted in skeleton deformations and resorptions

Fetal abnormalities

Abnormalities:

not specified

Overall developmental toxicity

Developmental effects observed:

not specified