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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Klimisch criteria

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): di-n-octylether
- Physical state: colorless liquid
- Purity: >99.9%
- Stability: stable at temperatures up to 40°C

Test animals / tissue source

Species:
rabbit
Strain:
other: Kleinrussen Chbb:HM
Details on test animals or tissues and environmental conditions:
according to guideline

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
application volume: 0.1 mL undiluted
Duration of treatment / exposure:
24 h
Observation period (in vivo):
72 h after instillation
Number of animals or in vitro replicates:
3
Details on study design:
according to guideline

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24 h/48 h/72 h
Score:
ca. 0.3
Reversibility:
fully reversible within: 72 h

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Only mild effects were observed that were fully reversible.. Therefore, the substance is considered to be slightly irritating.
Executive summary:

An aliquot of 0.1 ml aqueous solution of the test article was instilled by a single application for a 24h contact into the eyes of 3 rabbits. The eyes were scored 24, 48 and 72 hours after application. The 24/48/72 hours mean scores were 0 for the cornea, 0 for the iris, 0.33 for the conjunctival erythema and 0.11 for the conjunctival oedema. The conjunctiva reactions abated completely within 72 hours. In summary, only mild effects were observed that were fully reversible. Therefore, the substance is considered to be slightly irritating.