lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2012-07-30 to 2012-08-03. Study report date 2012-08-31

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

EC Number:

700-777-2

Cas Number:

667889-11-4

Molecular formula:

C11H9N2O5S2Li

IUPAC Name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Storage condition of test material: Ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 120 mg/L
- Sampling method: samples taken from the control and exposure test concentration at 0h and 96h

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION Required amount of test material was directly weighed into a glass vial and added to the dilution water in the test tank and stirred for 5 minutes.
- Evidence of undissolved material: Final solution clear and colourless

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Houghton Springs Fish Farm, Winterbourne Houghton, Blandford Forum, Dorset, DT11 0PD
- Feeding during test: None

ACCLIMATION
- Acclimation period: 40 days
- Acclimation conditions : same as test
- Type and amount of food: fed daily with commercial fish food
- Health during acclimation: 2 mortalities (<1%) recorded within 7 days prior to start of study

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

14.9 - 15.5 deg. C

pH:

7.15 - 7.79

Dissolved oxygen:

8.08 - 9.71 mg/L

Nominal and measured concentrations:

The measured concentration of test material at both the start and the end of the study was 124 mg/L. Based on the results of this analysis the nominal concentration was used for reporting the results.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass tanks
- Size: Test vessels with a working volume of 30L
- Aeration: Yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water coarsely filtered to remove particulate material, passed through activated carbon and dechlorinated using sodium thiosulphate. Salts added to maintain hardness levels and the treated water passed through a UV steriliser and filtered to 10um. Dilution water was delivered to header tank and set to nominal test temp of 15 +/-1 deg. C
- Particulate matter: <3 mg/L (water supply)
- Chlorine: Total <2 ug/L (water supply)
- Alkalinity: 26.0 mg/L as CaCO3 (water supply)
- Conductivity: 189.5 uS/cm (water supply)

OTHER TEST CONDITIONS
- Photoperiod: 16h light & 8h dark with 20 min. transition period.

EFFECT PARAMETERS MEASURED: Observations of mortality and symptoms of toxicity were made at 3, 24, 48, 72 & 96h

TEST CONCENTRATIONS
- Test concentration: 120 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Observations on body length and weight: 49-60 mm and 1.54-2.71 g
- Mortality of control: 0
- Other adverse effects control: none
- Abnormal responses: none
- Any observations that might cause a difference between measured and nominal values: Solutions were clear and colourless

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No mortalities were observed in the control or the 120 mg/L test concentration during this study. Therefore the 96h LC50 was determined to be >120 mg/L. It is concluded that 120 mg/L of the test substance did not have a significant toxic effect on the test organism, Oncorhynchus mykiss, in this study.

Executive summary:

Introduction

This study was performed to assess the acute toxicity of the test substance to rainbow trout Oncorhynchus mykiss. The method was designed to be compatible with:

OECD 203, Fish, Acute Toxicity Test. Adopted 17 July 1992.

Results & Conclusions

No mortalities were observed in the control or the 120 mg/L test concentration during this study. Therefore the 96h LC50 was determined to be >120 mg/L. It is concluded that 120 mg/L of the test substance did not have a significant toxic effect on the test organism, Oncorhynchus mykiss, in this study.