lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test dates 2011.09.08 to 2011.10.12. Report completed 2011.12.20

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study meets the criteria as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

EC Number:

700-777-2

Cas Number:

667889-11-4

Molecular formula:

C11H9N2O5S2Li

IUPAC Name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Totnes Sewage Treatment Works, Totnes, Devon, UK
- Storage conditions: At the laboratory the sludge was kept aerated at room temperature and maintained at pH 7.
- Preparation of inoculum for exposure: 6 days prior to exposure the activated sludge was centrifuged, washed and resuspended in the mineral medium and solids concentration determined. This sludge was then diluted in medium added to test bottles and stirred until required.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

50 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: According to OECD 301F guideline
- Test temperature:22 +/-1 C
- pH:7.4 +/-0.2 C prior to start of test
- Continuous darkness: yes

TEST SYSTEM
- Measuring equipment:Oxitop Respirometer (Wissenschaftlich-Technische Werkstatten, GmbH, Germany.) Oxygen uptake was measured every 112 min during the 28 day experimental period.



CONTROL AND BLANK SYSTEM
- Inoculum blank: Containing mineral medium only (no test or reference material)
- Reference: Sodium benzoate at 100mg/l

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/l

Results and discussion

Test performance:

Mean oxygen uptake of the inoculum blank was <60mg/l. The differences of extremes of replicate biodegradation values was <20% for both sodium benzoate and the test material after the 10 day window, at plateau and at day 28 as required in the OECD guideline.

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 3

Sampling time:

5 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

< 3

Sampling time:

10 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

< 3

Sampling time:

15 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

< 3

Sampling time:

20 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

< 3

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The sodium benzoate reference attained a maximum mean level of biodegradation of 62% and >60% biodegradation was attained during the 10 day window confirming that the activated sludge contained viable organisms.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

During this study the test material attained a maximum mean level of biodegradation of <3% therefore the test material cannot be classed as readily biodegradable.

Executive summary:

Introduction

This study was undertaken to determine the Ready Biodegradability of the test material by the measurment of Biochemical Oxygen Demand (BOD) in a manometric respirometry test. The study was designed to meet the following guideline:

OECD 301F Ready Biodegradability: Manometric Respirometry

Results and Conclusions

During this study the test material attained a maximum mean level of biodegradation of <3% and therefore was determined to be not readily biodegradable.