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EC number: 607-813-5
CAS number: 25830-77-7
Table 1 Concentrations of the test item in test medium
Time of sampling1 [hours]
Percentage of SS2 [%]
Analysed concentration [mg/L]
Relative to initial [%]
were stored in the freezer (≤ -15°C) until the day of analysis.
of a saturated solution prepared at a loading rate of 100 mg/L.
value, calculated by extrapolation of the calibration curve.
Table 2 Percentage inhibition of growth rate (total test period)
during the test
Phthaloyl-L-glutamic acid anhydride% SS prep. at 100 mg/L
() - Between brackets,
the measured concentration is given in mg/L.
* - effect was statistically significant
effect biologically not relevant (<10%)
Table 3 Percentage inhibition of growth rate at different time
intervals during the test
0 – 24 h
24 – 48 h
48 – 72h
() - Between brackets, the measured
concentration is given in mg/L.
Table 4 Percentage inhibition of yield during the test
study was performed to assess the effect of the test substance on the
growth rate and yield of fresh water algae ( Pseudokirchneriella
subcapitata ) after 72 hours of exposure. The study was conducted in
accordance with OECD Guideline for Testing of Chemicals No. 201 and GLP
under static conditions.
exponentially growing algal cultures were exposed to an untreated
control and the limit concentration, i.e. 100 mg/L. In addition, three
replicates per group were exposed to 1.0 and 10 mg of test substance per
litre in a combined limit/range-finding test. The initial algal cell
density was 104
cells/mL. The total
exposure period was 72 hours. Samples from the control and the highest
concentration were taken and analysed at the start, after 24 and 72
hours of exposure. Measured concentration at t=0 was 88 mg/L.
Measured concentrations during the test ranged from 87 - 99 % of initial
loading rates, therefore it was concluded that the test substance
remained stable during the exposure period and the initial measured
concentration was used to determine the effect parameters.
at the highest concentrations, an inhibition of growth rate and an
inhibition of yield were observed. The inhibitions were statistically
significant (p < 0.05). However, the inhibition of growth rate was
biologically not relevant (< 10%). The NOEC for growth rate was
therefore determined to be 88 mg/L. The NOEC for yield was determined to
be < 88 mg/L. EC50 for inhibition of growth rate and inhibition of yield
in algae after 72 hours of exposure could not be calculated because the
test item proved to be non-toxic (EC50> maximum soluble concentration
tested). EC50 for both growth rate and yield were determined to be > 88
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