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EC number: 607-813-5
CAS number: 25830-77-7
Table 1 Concentrations of the test item in test medium
Time of sampling1 [hours]
Percentage of SS2 [%]
Analysed concentration [mg/L]
Relative to initial [%]
were stored in the freezer (≤ -15°C) until the day of analysis.
of a saturated solution prepared at a loading rate of 100 mg/L.
Table 2 Number of introduced daphnids and incidence of immobility
in the test
Phthaloyl-L-glutamic acid anhydride % SS prep. at 100 mg/L
( ) between brackets: number of daphnia observed trapped at the
surface of the test solutions. These organisms were reimmersed into the
respective solutions before recording of mobility.
This study was performed to assess the
effect of the test substance on the mobility of Daphnia magna after
48 hours. The study was conducted in accordance with OECD Guidline for
Testing of Chemicals No. 202 and GLP under static conditions.
In a combined limit/range-finding test, 20 Daphnia
magna (less than 24 hours old) were exposed to the test substance
for 48 hours in all-glass, open vessels. The exposure was performed in
dark.Test solutions were prepared at
nominal concentrations of 1.0, 10 and 100 mg/L. The incidence of
immobilisation was recorded for each test and control group at 24 hours
and at 48 hours. Samples from the controls and the highest
concentrations were taken and analysed to determine the test
concentrations. The measured concentration at t=0 for the highest
concentration was 85 mg/L and remained stable during the exposure period
(95% of initial at t=48).
No immobility was observed at the controls
or any of the concentrations. The 48h-EC50 was beyond the range tested,
i.e. exceeded an analytically confirmed nominal concentration of 100
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