Registration Dossier
Registration Dossier
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EC number: 228-705-0 | CAS number: 6330-25-2
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro Gene Mutation Test:
The test substance 2 -cyano-N-methylacetamide was investigated according to OECD TG 471 for its potential to cause gene mutation in Salmonella typhimurium strains (TA98, TA100, TA1537, TA1535 and TA1538) and Escherichia coli WP2 uvrA (BASF 2001). The Standard plate test (SPT) as well as the Preincubation test (PIT) were performed with test concentrations of 125 µg - 31,250 µg/plate. Due to the good water solubility of the substance, aqua dest. was used as vehicle. The test was performed with and without a metabolic activation system. For this purpose, the mammalian liver post-mitochondrial fraction (S-9) was prepared (Aroclor induced rat liver S-9 mix). Results: No toxic effects were reported. Up to the highest investigated dose, no relevant increase of the revertant colony numbers was obtained in any Salmonella typhimurium strain as well as in the E.coli strain in comparison with the corresponding controls. In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test material caused neither base-pair substitutions, nor frameshift mutations. Therefore the test results revealed no indication of gene mutagenic activity.
Short description of key information:
In an in-vitro gene mutation study (Ames test OECD TG 471, GLP) the test substance did not induce genetic toxicity (BASF 2001).
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Dangerous Substance Directive (67/548/EEC)
The available studies is incomplete for classification purposes under 67/548/EEC. The Ames test did not indicate a mutagenic potential. As a result the substance is not considered to be classified for mutagenicity under Directive 67/548/EEC, as amended for the 28th time in Directive 2001/59/EC.
Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are not complete for classification purposes under Regulation 1272/2008. The Ames test did not indicate a mutagenicity hazard. As a result the substance is not considered to be classified for mutagenicity under Regulation (EC) No. 1272/2008, as amended for the second time in Directive (EC 286/2011).
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