Registration Dossier
Registration Dossier
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EC number: 228-705-0 | CAS number: 6330-25-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-cyano-N-methylacetamide
- EC Number:
- 228-705-0
- EC Name:
- 2-cyano-N-methylacetamide
- Cas Number:
- 6330-25-2
- Molecular formula:
- C4H6N2O
- IUPAC Name:
- 2-cyano-N-methylacetamide
- Details on test material:
- - Name of test material (as cited in study report): Methylcyanamin in LSG. B. 100%
- Analytical purity: 15% (an aqueous solutions was tested)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: laboratory wastewater plant
- Initial biomass concentration: 1g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- Nominal:
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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