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EC number: 228-705-0 | CAS number: 6330-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without certified GLP compliance
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Fed.Reg.38, No.187, Para.1500.41, S.27019 (1973-09-27)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-cyano-N-methylacetamide
- EC Number:
- 228-705-0
- EC Name:
- 2-cyano-N-methylacetamide
- Cas Number:
- 6330-25-2
- Molecular formula:
- C4H6N2O
- IUPAC Name:
- 2-cyano-N-methylacetamide
- Details on test material:
- - Name of test material: N-Methylcyanacetamid
- Substance number: 81 / 97
- Physical state: 80 % aqueous preparation
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Firma Gaukler, Offenbach / Main
- Weight at study initiation (mean): male: 2.89 kg / female: 3.01 kg
- Housing: Stainless steel cage with wire shelves, ground area: 40 x 51 cm
- Diet (ca. 130 g/animal): Ovator Solikanin 4mm, Muskator-Werke Düsseldorf
- Water (ca. 250 mL/animal): VE-water on week days, tapwater on holidays
- Acclimation period: about 8 days
- Shaving: at least 15 h before application
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): ca. 30 - 70
- Photoperiod (hrs dark / hrs light): 12 / 12
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- water
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL:
- Concentration (if solution): 80 % - Duration of treatment / exposure:
- Single application
- Observation period:
- 7 d
- Number of animals:
- 6 (4 males and 2 females)
- Details on study design:
- TEST SITE
- Area of exposure: upper third of the back or flank region
-Applikation: The substance (80 % grind) is applied onto the test lobe as an ~ 0.5 mm thick layer.
REMOVAL OF TEST SUBSTANCE
- Washing: not performed
FURTHER INVESTIGATIONS
Sacrified animals pathological examined afterwards and especially investigated for necrosis.
Results and discussion
In vivo
Results
- Irritation parameter:
- other: Primary Irritation Index
- Basis:
- other: Sum of all single stimulus values
- Time point:
- other: 24h, 72h
- Score:
- 1
- Reversibility:
- no data
- Remarks on result:
- other: No erythema and edema were observed at the 7 day reading. One animal showed scuffing.
- Irritant / corrosive response data:
- Only one animal showed at the scarified skin a slight redness 24 h after test substance application. All other investigations were recorded as "doubtful" (intact and scarified skin, redness and edema).
- Other effects:
- Scuffing was observed at one animal after 7 days at the intact as well as at the scarified skin.
Any other information on results incl. tables
Table 1: Results Skin Irritation
(according to FED. REG. 38, NO. 187, PARA. 1500.41, S. 27019 (1973 -09 -27)) |
|||||||||
Animal |
1 |
2 |
3 |
4 |
5 |
6 |
|||
Number of Animal |
0335 |
0354 |
0384 |
0326 |
0293 |
0288 |
|||
Weight (kg) |
3.59 |
3.09 |
2.92 |
2.88 |
2.60 |
2.49 |
|||
Sex |
male |
female |
female |
male |
male |
male |
|||
|
|
||||||||
Time |
Animal |
Intact Skin |
Scarified Skin |
Single Stimulus Value |
|||||
R |
OE |
Symptoms |
R |
OE |
Symptoms |
||||
24 h |
1 |
1 |
0 |
|
1 |
0 |
|
1.0 |
|
2 |
1 |
0 |
1 |
1 |
1.5 |
||||
3 |
1 |
1 |
2 |
1 |
2.5 |
||||
4 |
1 |
0 |
1 |
1 |
1.5 |
||||
5 |
1 |
0 |
1 |
1 |
1.5 |
||||
6 |
1 |
0 |
|
1 |
0 |
Redness |
1.0 |
||
72 h |
Mean value |
1.0 |
0.2 |
|
1.2 |
0.7 |
|
1.5 |
|
1 |
0 |
0 |
1 |
0 |
0.5 |
||||
2 |
0 |
0 |
0 |
0 |
0. |
||||
3 |
1 |
0 |
1 |
0 |
1.0 |
||||
4 |
0 |
0 |
1 |
0 |
0.5 |
||||
5 |
0 |
0 |
1 |
0 |
0.5 |
||||
6 |
0 |
0 |
|
0 |
0 |
|
0. |
||
7 d |
Mean value |
0.2 |
0. |
|
0.7 |
0. |
|
0.4 |
|
1 |
0 |
0 |
0 |
0 |
0. |
||||
2 |
0 |
0 |
0 |
0 |
0. |
||||
3 |
0 |
0 |
Scuffing |
0 |
0 |
Scuffing |
0. |
||
4 |
0 |
0 |
0 |
0 |
0. |
||||
5 |
0 |
0 |
0 |
0 |
0. |
||||
6 |
0 |
0 |
0 |
0 |
0. |
||||
|
Mean value |
0 . |
0 . |
|
0. |
0. |
|
0. |
|
11.5 / 12 = 1.0 |
|||||||||
Primary Irritation Value = 1.0 |
R = redness
OE = edema
Calculation of the Irritation Index:
Single Stimulus Value = (R + OE of intact and scarified skin) / 2
The Primary Irritation Index is the sum of all single stimulus values of all animals and the time points of 24 h and 72 h divided by 2 x the number of animals.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Only one animal showed at the scarified skin a slight redness 24 h after test substance application. All other investigations were recorded as "doubtful" (intact and scarified skin, redness and edema). The calculation of the Primary Irritation Index was performed according to Draize (1965), resulting in a value of 1, thus indicating a no irritation potential for the skin.
- Executive summary:
The test substance 2 -cyano-N-methylacetamide was investigated for its potential to cause irritation on the rabbit skin according to Fed.Reg.38, No.187, Para.1500.41, S.27019 (1973) with one single application. At least 15 h before application the rabbits (6 animals, 4 males and 2 females) were shaved and the 80 % test solution was applied to intact and scarified skin under occlusion. The test duration was 7 days, and the sacrified animals were pathologically examined, especially in context of necrosis. Only one animal showed at the scarified skin a slight redness 24 h after test substance application. All other investigations were recorded as "doubtful" (intact and scarified skin, redness and edema). Scuffing was observed at one animal after 7 days at the intact as well as at the scarified skin. The Primary Irritation Index was calculated according to the scheme of Draize (1965), resulting in a value of 1.0, indicating no irritation potential of the test substance.
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