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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Justification for type of information:
Data is from study report.
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively.
- Sampling method: The sample was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg / l and 250 mg / l, a slight precipitate was seen at the end of the test.

Vehicle:
not specified
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Species: Pseudomonas putida
- Strain: DSM 50026
- Growth period: 7 ± 1 h
Test type:
not specified
Water media type:
not specified
Remarks on exposure duration:
Total duration of study was not mentioned.
Test temperature:
297K ± 1K
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Nominal volume and net volume of the test vessel was 300 and 100 ml, respectively.
- No. of vessels per concentration (replicates): 1
- Nutrients provided for bacteria:
0.5 ml nutrient concentration I (20 subject)
0.5 ml nutrient concentration II (20 subject)

TEST MEDIUM / WATER PARAMETERS: AK-Medium DIN 38412 Part 8 draft was used as a test medium for the study.

TEST CONCENTRATIONS
- Test concentrations: Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively.
Reference substance (positive control):
not specified
Key result
Dose descriptor:
EC10
Effect conc.:
62.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Other details not known
Key result
Dose descriptor:
EC50
Effect conc.:
242.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Other details not known

Experimental results

Conc. In mg/l

E 436 nm

Percentage of the control

Inoculated

Uninoculated

Difference

Control inoculated

 

0.354

 

 

0.98

1.95

3.91

7.91

15.265

31.25

62.5

125

250

500

0.39

0.354

0.408

0.408

0.412

0.375

0.334

0.286

0.226

0.19

0.008

0.005

0.005

0.002

0.004

0.006

0.016

0.028

0.054

0.107

0.382

0.349

0.403

0.0406

0.408

0.369

0.318

0.258

0.172

0.083

107.99

98.73

113.93

114.71

115.28

104.38

89.89

72.84

48.51

23.48

EC 10 = 62.3 mg/l

EC 50 = 242.4 mg/l

EC 90 = ---

 

Conc.

Individual values

Sample inoculated

Net values

Standard deviation

Variance in %

0.98

0.398

0.39

0.385

0.386

 

0.008

0.382

0.006

1.52

1.95

0.284

0.415

0.348

0.369

 

0.005

0.349

0.054

15.37

3.91

0.444

0.356

0.397

0.434

 

0.005

0.403

0.04

9.81

7.91

0.313

0.4

0.473

0.444

 

0.002

0.406

0.07

17.12

15.625

0.372

0.456

0.388

0.43

 

0.04

0.408

0.038

9.34

31.25

0.337

0.457

0.339

0.367

 

0.006

0.369

0.056

15.08

62.5

0.3

0.315

0.355

0.365

 

0.018

0.318

0.031

9.35

125

0.226

0.34

0.262

0.314

 

0.028

0.258

0.851

17.95

250

0.207

0.234

0.236

0.225

 

0.054

0.172

0.013

5.87

500

0.186

0.202

0.182

0.19

 

0.107

0.083

0.009

4.55

 

Validity criteria fulfilled:
not specified
Conclusions:
Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.
Executive summary:

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K. The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Description of key information

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Key value for chemical safety assessment

EC50 or LC50 for microorganisms:
242.4 mg/L
EC10, LC10 or NOEC for microorganisms:
62.3 mg/L

Additional information

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.