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Ecotoxicological information

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Description of key information

Short term toxicity to fish

Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology (Sustainability Support Services (Europe) AB, Report no. 10F0135/875091, 1987). The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively. The study was based on the effects of the test compound4-amino-N-(4-aminophenyl)benzene-1-sulfonamide(CAS no. 16803-97-7) on Leuciscus idus (Golden Orfe) in a static fresh water system at a temperature of 20 ± 1°C, pH of about 7.6, Total hardness of 2.5 mmol/l and Oxygen content > 60% of maximum saturation with a photoperiod of 16 hr light and 8 hr darkness. Test chemical concentration used for the study were 250 and 500 mg/l, respectively. Chloroacetamide was used as a positive control. Leuciscus idus (Golden Orfe) of 5.1 cm in length (ranges from 4.8 to 5.7 cm), 1.8 gm in weight (ranges from 1.4 to 2.7 gm)was used as a test organism. The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air. The test organism was kept for housing and adapted was about 2 weeks and fed with growth feed F/B 50, Ad libitum. Test was performed in glass aquarium. The size of the test vessel was 30 cm X 22 cm X 24 cm and volume 10 ml. Test system was slightly aerated. No. of organisms used per conc. was 10 and biomass loading rate consists of 1.8 fish/l test water. Reconstituted freshwater water was used as a dilution water during the study. Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC5 and the LC95 was calculated using the probit analysis after hours 1, 24, 48, 72 and 96, respectively. The 48 hr LC50 for the positive control chloroacetamide was determined to be 32 mg/l. No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively. Thus, based on the LC50 value, it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline (Sustainability Support Services (Europe) AB, Report no. 1/0164/2/87-164/87, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Daphnia magnain a static fresh water system at a temperature of 292 -294 K, pH range 7.7 – 8.3, hardness of water was 2.70 ± 0.50 mmol/l and oxygen content was > 2 mg/l, respectively. Daphnia magna Straus of 6 -24 h old was used as a test organism obtained from the Institute National Research Chemical Applied, France. Test animals were fed bewer’s yeast 1X every day green algae culture. The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.Centrifuge glass with flattened bottom was used as a test vessel. The test water used is tap water, which is passed through a 6-micron filter cartridge and through an activated carbon filter to remove any chlorine that may be present. The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization. No. of organisms used per vessel was 5 and total no. of organisms/conc. were 20, respectively. The test was performed in 4 repilcates with a light intensity of 16: 8 hours Day : Night. Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.EC50 after 24 and 48 hrs was determined using the statistical probit and moving average method. At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively. Thus, based on the EC50 value (48 hr = 56.09 mg/l), it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Toxicity to microorganisms

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Additional information

Short term toxicity to fish

Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology (Sustainability Support Services (Europe) AB, Report no. 10F0135/875091, 1987). The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively. The study was based on the effects of the test compound4-amino-N-(4-aminophenyl)benzene-1-sulfonamide(CAS no. 16803-97-7) on Leuciscus idus (Golden Orfe) in a static fresh water system at a temperature of 20 ± 1°C, pH of about 7.6, Total hardness of 2.5 mmol/l and Oxygen content > 60% of maximum saturation with a photoperiod of 16 hr light and 8 hr darkness. Test chemical concentration used for the study were 250 and 500 mg/l, respectively. Chloroacetamide was used as a positive control. Leuciscus idus (Golden Orfe) of 5.1 cm in length (ranges from 4.8 to 5.7 cm), 1.8 gm in weight (ranges from 1.4 to 2.7 gm)was used as a test organism. The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air. The test organism was kept for housing and adapted was about 2 weeks and fed with growth feed F/B 50, Ad libitum. Test was performed in glass aquarium. The size of the test vessel was 30 cm X 22 cm X 24 cm and volume 10 ml. Test system was slightly aerated. No. of organisms used per conc. was 10 and biomass loading rate consists of 1.8 fish/l test water. Reconstituted freshwater water was used as a dilution water during the study. Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC5 and the LC95 was calculated using the probit analysis after hours 1, 24, 48, 72 and 96, respectively. The 48 hr LC50 for the positive control chloroacetamide was determined to be 32 mg/l. No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively. Thus, based on the LC50 value, it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline (Sustainability Support Services (Europe) AB, Report no. 1/0164/2/87-164/87, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Daphnia magnain a static fresh water system at a temperature of 292 -294 K, pH range 7.7 – 8.3, hardness of water was 2.70 ± 0.50 mmol/l and oxygen content was > 2 mg/l, respectively. Daphnia magna Straus of 6 -24 h old was used as a test organism obtained from the Institute National Research Chemical Applied, France. Test animals were fed bewer’s yeast 1X every day green algae culture. The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.Centrifuge glass with flattened bottom was used as a test vessel. The test water used is tap water, which is passed through a 6-micron filter cartridge and through an activated carbon filter to remove any chlorine that may be present. The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization. No. of organisms used per vessel was 5 and total no. of organisms/conc. were 20, respectively. The test was performed in 4 repilcates with a light intensity of 16: 8 hours Day : Night. Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.EC50 after 24 and 48 hrs was determined using the statistical probit and moving average method. At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively. Thus, based on the EC50 value (48 hr = 56.09 mg/l), it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Toxicity to microorganisms

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

On the basis of above mentioned available information for short term toxicity to fish, aquatic invertebrates and toxicity to aquatic algae and cyanobacteria, test substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.