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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 25 µl, 50 µl, 100 µl , 200 µl and 400 µl of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L & 50 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 0.234 cm
- Weight at study initiation (mean and range, SD): average 2.8 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12-hour darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
32.5 mg of CaCO3
Test temperature:
23-24°C
pH:
7.9
Dissolved oxygen:
5.77 mg/L
Nominal and measured concentrations:
6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L, 200 mg/L & 400 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12 hours darkness
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: highest concentration at which no mortality was observed
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: lowest concentration at which 100% mortality was observed

8.1. Mortality and symptoms

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

-

-

-

-

6.25

8

-

-

-

-

12.5

8

-

-

-

-

25

8

-

-

-

-

50

8

-

-

-

-

100

8

8

-

-

-

200

8

8

-

-

-

400

8

8

-

-

-

Nominal conc. (mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS

NS

NS

NS

6.25

8

M

M

M

M

12.5

8

M

M

M

M

25

8

M

M

M

M

50

8

M

M

M

M

100

8

-

-

-

-

200

8

-

-

-

-

400

8

-

-

-

-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

8.2. pH and oxygen content:

Nominal conc. (mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

5.13

5.29

2.35

5.15

6.25

5.22

5.31

5.48

5.2

12.5

5.30

5.38

5.51

5.40

25

5.3

5.38

5.51

5.40

50

5.35

5.40

5.51

5.40

Nominal conc. (mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

5.55

6.12

6.41

6.36

6.25

4.84

5.83

5.84

5.61

12.5

4.78

5.49

5.62

5.66

25

5.14

5.71

5.93

5.66

50

5.12

5.48

5.71

4.83

8.3. Test temperature

Nominal conc. (mg/l)

Temperature (°C) after

24h

48h

72h

96h

0 (control)

24

25

22

23

6.25

24

25

22

23

12.5

24

25

22

23

25

24

25

22

23

50

24

25

22

23

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material. on Zebra Fish Danio rerio was determine to be > 50 mg/L<100 mg/l.
Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 25 µl, 50 µl, 100 µl , 200 µl and 400 µl   of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L & 50 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be > 50 mg/L<100 mg/l . Based on the LC50, it can be consider that the chemical was and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 25 µl, 50 µl, 100 µl , 200 µl and 400 µl   of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L & 50 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be> 50 mg/L<100mg/l . Based on the LC50, it can be consider that the chemical was and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
50 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 25 µl, 50 µl, 100 µl , 200 µl and 400 µl   of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L & 50 mg/L, respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be> 50 mg/L<100mg/l . Based on the LC50, it can be consider that the chemical was and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.