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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of aquatic invertebrate for the test chemical :
2) Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.
3) Short term toxicity to aquatic invertebrate was evalauted for test material
4)Short term toxicity to aquatic invertebrates was performed acording to the OECD guideline 202 in a static system.
Analytical monitoring:
not specified
Details on test solutions:
2) The solution of colourless liquid of purity 99.62% 100 mg/l was prepaired by dissolving the substance into reconstitued test water.
4)The stock solution 200mg/l was prepared by dissolving white powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
Test organisms (species):
Daphnia magna
Details on test organisms:
2) and 4) TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Test temperature:
2) and 4) 20±1°C
pH:
2) Sample : pH = 7.4 changed to pH= 8.0 during the test
Control pH = 8.0 did not changed during the test
2) higher than 7.9 mg/L at the end of test

4)higher than 8.5 mg/L at the end of test
4)Test: 7.9 changed to pH=7.7 during the test,
Control: 8 changed to pH=7.9 during the test,
Nominal and measured concentrations:
2)100 mg/l
4)0, 50, 70,100,140 and 200 mg/L
Details on test conditions:
1)
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Duration:
48 h
Dose descriptor:
other: Inhibition percentage [I%]
Effect conc.:
4 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 24 out of 25 daphnids were observed to be mobile in the sample
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
230 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
189 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CL; 140.4-254.9
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical is not likely to be toxic to aquatic invertebrtae in the concentration range of 100-230 mg/l
Executive summary:

Data available for the structurally and functionally similar read across substance has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

Aim of this study was to assess the short term toxicity of to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The solution of colourless liquid of purity 99.62% 100  mg/l was prepaired by dissolving the substance into reconstitued test water.100 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 The inhibition perccentage [%] for the test substance , in Daphnia magna was determined to be 4.0% on the basis of mobility inhibition effects in a 48 hour study.24 out of 25 daphnids were observed to be mobile in the sample. Based on the [I%] value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot as per the CLP criteria.

The above eperimental data was further supported by data summaried in authoritative database further explaining the toxicity of structurally similar read across substance for its potential to cause toxicity to aquatic invertebrate.

Short term toxicity to aquatic invertebrate was evaluated for 48 h according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The EC50 value after 48 h of test material exposure was observed to be 230 mg/l.

Based on the above effect concentration (EC50) ,it can be considered that test substance is not toxic for fish and can not be classified as per CLP criteria.

Experimental data available for functionally similar read across substance was used to further support the toxicity of aquatic invertebrate, aim of this study was to assess the short term toxicity of test substance to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 10mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 50, 70,100,140 and 200 mg/L mg/L nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 189.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

Based on the median effective concentration value, it is concluded that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Description of key information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across substance has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

Aim of this study was to assess the short term toxicity of to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The solution of colourless liquid of purity 99.62% 100  mg/l was prepaired by dissolving the substance into reconstitued test water.100 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 The inhibition perccentage [%] for the test substance , in Daphnia magna was determined to be 4.0% on the basis of mobility inhibition effects in a 48 hour study.24 out of 25 daphnids were observed to be mobile in the sample. Based on the [I%] value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot as per the CLP criteria.

The above eperimental data was further supported by data summaried in authoritative database further explaining the toxicity of structurally similar read across substance for its potential to cause toxicity to aquatic invertebrate.

Short term toxicity to aquatic invertebrate was evaluated for 48 h according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The EC50 value after 48 h of test material exposure was observed to be 230 mg/l.

Based on the above effect concentration (EC50) ,it can be considered that test substance is not toxic for fish and can not be classified as per CLP criteria.

Experimental data available for functionally similar read across substance was used to further support the toxicity of aquatic invertebrate,aim of this study was to assess the short term toxicity of test substance to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 10mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 50, 70,100,140 and 200 mg/L mg/L nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 189.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

Based on the median effective concentration value, it is concluded that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
189.2 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Data available for the structurally and functionally similar read across substance has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

Aim of this study was to assess the short term toxicity of to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The solution of colourless liquid of purity 99.62% 100  mg/l was prepaired by dissolving the substance into reconstitued test water.100 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 The inhibition perccentage [%] for the test substance , in Daphnia magna was determined to be 4.0% on the basis of mobility inhibition effects in a 48 hour study.24 out of 25 daphnids were observed to be mobile in the sample. Based on the [I%] value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot as per the CLP criteria.

The above eperimental data was further supported by data summaried in authoritative database further explaining the toxicity of structurally similar read across substance for its potential to cause toxicity to aquatic invertebrate.

Short term toxicity to aquatic invertebrate was evaluated for 48 h according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The EC50 value after 48 h of test material exposure was observed to be 230 mg/l.

Based on the above effect concentration (EC50) ,it can be considered that test substance is not toxic for fish and can not be classified as per CLP criteria.

Experimental data available for functionally similar read across substance was used to further support the toxicity of aquatic invertebrate,aim of this study was to assess the short term toxicity of test substance to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 The stock solution 10mg/l was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 50, 70,100,140 and 200 mg/L mg/L nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 189.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

Based on the median effective concentration value, it is concluded that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.