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EC number: 248-273-7 | CAS number: 27157-94-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- FEB 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes
- Remarks:
- Except chemical analysis (carried our as accredited analysis but not in compliance with GLP principles)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, industrial (adaptation not specified)
- Details on inoculum:
- Secondary effluent from a municipal wastewater treatment plant in Usserød, Denmark, which predominantly receives domestic sewage.
0.5 mL of secondary effluent per litre of test medium. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.4 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7.1
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7.9
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 9
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7.9
- Sampling time:
- 28 d
- Key result
- Parameter:
- COD
- Value:
- 250 other: mg/L
- Remarks on result:
- other: COD determined in stock solution of 120.1 mg test product/L
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Biodegradation in water screening test shows that test item is not readily biodegradable under the test conditions within the 28-day exposure period.
- Executive summary:
The ready biodegradability of the test item was examined according to OECD Guideline 301D, under GLP compliance. BOD of 9.0 ±1.4 % of the ThOD was reached within 28 days (after 21 days). After 28 days the BOD was measured to be 7.9 ± 0.2 % of the ThOD. These results give a conclusion that test item is not readily biodegradable.
Reference
Biodegradation of test substance and reference substance tested separately and in combination.
| Time (Days) | Test product "Danafloat TM 070 (Cresyl-P1)" (% of ThOD) | Activity control (sodium benzoate) (% of ThOD) | Toxicity control "Danafloat TM 070 (Cresyl-P1)"+sodium benzoate (% of ThOD) |
| 7 | 7.1 | 65.2 | 29.5 |
| 14 | 7.9 | 72.6 | 36.6 |
| 21 | 9.0 | 76.9 | 34.6 |
| 28 | 7.9 | 71.2 | 31.9 |
Description of key information
The ready biodegradability of the test item was examined according to OECD Guideline 301D, under GLP compliance. BOD of 9.0 ±1.4 % of the ThOD was reached within 28 days (after 21 days). After 28 days the BOD was measured to be 7.9 ± 0.2 % of the ThOD. These results give a conclusion that test item is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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