[No public or meaningful name is available]

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 27-31, 2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids C10-20, reaction product with diethylenetriamine

EC Number:

940-308-0

IUPAC Name:

Fatty acids C10-20, reaction product with diethylenetriamine

Test material form:

liquid: viscous

Details on test material:

Test substance: Fatty acids, C10-20 neo, reaction product with diethylenetriamine
Old name of the test material: MK195K
Solubility: Immiscible with water, soluble in aromatic hydrocarbons
Appareance: brown viscous liquid
Odor: Aromatic
Storage: room temperature

Specific details on test material used for the study:

Old names of the test substance: Naphthenic acids, reaction products with diethylenetriamine; MK195K.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

< 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 8

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

1.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 9

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 10

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Upon addition of the product to the test media it was observed that Fatty acids, C10 -20 neo, reaction product with diethyletriamine was completely insoluble in water. EC50 for the substance (WSFs) is < 0.25 mg/l.

Applicant's summary and conclusion

Conclusions:

The scope of the study is to evaluate the effects water soluble fractions of Fatty acids, C10 -20 neo, reaction product with diethylenetriamine on the growth of Selenastrum capricornotum. Chemical analysis of the water soluble fractions was not performed. All values were based upon nominal concentrations of the substance in test water.
The test substance is an UVCB substance, insoluble in water; upon addition to seawater in the mixing jars the majority of the material would float (relative density: 0.83-1.01) or be caught up in the stirring vortex. Furthermore it has surfactant properties, tends to accumulate at the interfaces and to form aggregates, such as micelles, in the bulk aqueous phase.
These characteristics do not allow to to conduct tests appropriately to obtain exposure of the test species.
The FND ( Fatty Nitrogen Derived) Amides chemicals should be considered to be toxic to aquatic species under conditions that allow contact of the chemicals with the organisms. This toxicity is common not only to the FND Amides chemicals but also to the FND Amines, Cationics, and Nitriles Categories. Further, the high toxicity is consistent with that of a large body of surfactants (e.g. alcohol ethoxylates, alkylphenol ethoxylates, sodium lauryl sulfate, and others).