Benzaldehyde, 2-hydroxy-5-nonyl-, oxime, branched

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No solvent control was tested.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Lix 860 N I ("high oxime"); Benzaldehyde, 2-hydroxy-5-nonyl-,oxime
In the study the wrong CAS-no. 9000-22-43-7 is stated. However, the name given in the report is a synomyn for CAS 174333-80-3
- Physical state: brown viscous liqid
- Analytical purity: 90% (84 % AS+ 5 % Nonylphenol + 1 % Etheraldehyde)
- Solvent: 10 % Kerosene
- Purity test date: 1998-07-15
- Lot/batch No.: T-7203
- Expiration date of the lot/batch: 2003-07-31

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Loadings of 1 to 100 mg test substance/L (nominal) were stirred in 2 to 5 L of test medium for about 24 h. On the surface of the stock solutions and the glass beakers brown particles and oil drops were observed. In the range of 1 to 10 mg/L the stock solutions were clear, beginning at the concentration of 30 mg/L the solutions showed a homogeneous turbidity. After a settling time of about 2 h all solutions were filtered through a fast filtrating folded filters.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Bio International BV, NJ Hoorn, Netherlands
- Age at study initiation (mean and range, SD): < 24h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 °C

pH:

8

Dissolved oxygen:

> 90% saturation

Nominal and measured concentrations:

Nominal: 1, 3, 10, 30 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beakers with 50 mL test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: O2 content, temperature and pH were measured at the beginning and at the end of the test.

EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 24 and 48h test duration.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks:

(WAF)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

ca. 3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Details on results:

Toxicity was determined in water accommodated fraction (WAF) of the test substance. WAF was obtainedafter about 24h equilibration of nominal loadings of 1 to 100 mg/L of the substance andsubsequent removal of the undissolved moieties. Mean recovery (by HPLC) was 4.9%.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50/LC50: 1.5 mg/L = within target range of 0.9 - 1.9 mg/L

Validity criteria fulfilled:

yes

Conclusions:

In a GLP study according to the EU Method C.2, the LC50 for acute toxicity to Daphnids of the test substance is determined to be 2.7 mg a.i./L.

Executive summary:

The acute toxicity of the test substance to daphnids was evaluated in a study according to EU Method C.2 under GLP conditions.

In a static study Daphnia magna were exposed for 48h. test concentrations of 1 to 100 mg/L were applied as loading rate WAFs. Analytical monitoring was not performed. All validity criteria were fulfilled. The EL50 is determined to be 2.7 mg a.i./L.

Description of key information

EL50 (48h) = 2.7 mg/L (nominal, WAF, active ingredient) for Daphnia magna (EU method C.2)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.7 mg/L

Additional information

One study investigating the short-term toxicity of the test substance to aquatic invertebrates is available. The study was performed according to EU Method C.2 under GLP conditions using Daphnia magna as test organism (Kirch 1998). Test concentrations of 1 to 100 mg/L were applied as loading rate WAFs. Analytical monitoring was not performed. All validity criteria were fulfilled. The EL50 is determined to be 2.7 mg a.i./L.