2,2'-butyliminodiethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-03-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (source of inoculum industrial instead of domestic wwtp)

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, industrial

Details on inoculum:

- Source: activated sludge of an wwtp treating industrial sewage (BASF AG)
- Initial biomass concentration: dry matter: 1 g/L

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

20-25 °C

Nominal and measured concentrations:

nominal: 0 (control), 363, 727 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 727 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Respiration inhibition:

Test results after an incubation of 30 min:

Test concentration	Respiration rate	Respiration change*
[mg/L]	[mg O2/L/h]	[%]
blank	27
363	36	-33
727	36	-33

*: negative values represent inhibition of respiration; positive values represent promotion of respiration

Validity criteria fulfilled:

yes

Conclusions:

EC20 > 727 mg/L (nominal concentration)

Executive summary:

The toxicity of Butyldiethanolamine towards aquatic microorganisms was determined according to the international standard ISO 8192 (BASF, 1987). Activated sludge of an wwtp treating industrial sewage (BASF AG) with an initial biomass concentration of 1 g dw/L was exposed to the test substance for 30 minutes. Test concentrations of 0 (control), 363 and 727 mg/L were applied. Based on the respiration rate, the EC20 was determined to be > 727 mg/L (nominal concentration).

Description of key information

Butyldiethanolamine_ISO 8192_BASF-1987: EC20 (30 min) > 727 mg/L (nominal concentration)

Additional information

The toxicity of the registered substance Butyldiethanolamine towards aquatic microorganisms has been experimentally determined.

In addition, a study performed with the read-across substance DBEA serves as supporting information.

 

The toxicity of the source substance Butyldiethanolamine towards aquatic microorganisms was determined according to the international standard ISO 8192 (BASF, 1987). Activated sludge of a wwtp treating industrial sewage (BASF AG) with an initial biomass concentration of 1 g dw/L was exposed to the test substance for 30 minutes. Test concentrations of 0 (control), 363 and 727 mg/L were applied. Based on the respiration rate, the EC20 was determined to be > 727 mg/L (nominal concentration).

 

Furthermore, data on its structural analogue Dibutylethanolamine (DBEA, CAS 102-81-8) is taken to evaluate this endpoint (please refer also to read-across statement attached in IUCLID Section 13).

The toxicity of the read-across substance Dibutylaminoethanol (CAS 102-81-8) towards aquatic microorganisms was determined according to OECD Guideline 209 in compliance with GLP (BASF AG, 2005). Substance concentrations of 0 (blank control) and 1000 mg/L were tested (limit test). As inoculum, domestic activated sludge was used. Microorganisms were exposed to the test substance for 30 min. As reference substance, 3,5-dichlorophenol was selected. The respective EC50 (30 min) value of 3,5-dichlorophenol was in the range of 5-30 mg/L. For the test substance, an EC20 (30 min) value of > 1000 mg/L based on the respiration rate was determined.