2,2'-butyliminodiethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-04-13 to 1987-04-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed information

Qualifier:

according to guideline

Guideline:

other: DIN 38412

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden Orfe
- Source: Fischzucht Paul Eggers, Hohenwestedt
- Length at study initiation (length definition, mean, range and SD): 6.4 (6.0 - 6.9) cm
- Weight at study initiation (mean and range, SD): 3.5 (3.0 - 4.1) g
- Food type: Grow Feed F/B 50
- Amount: ad libitum

ACCLIMATION
- Acclimation period: 2 months
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): 0%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20°C

pH:

8.0 - 9.3 (control and all treatments without pH adjustment)
7.6 - 8.0 (464 mg/L, pH adjusted)

Dissolved oxygen:

7.1 - 9.1

Nominal and measured concentrations:

0 (control), 100, 147, 215, 316, 464, 464 (pH adjusted) mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm³)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 L volume
- Aeration: slightly
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: fully dimineralized tap water
- Ca/mg ratio: 4:1
- Conductivity: 10 µMHO
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: yes, additionally in the highest concentration
- Photoperiod: 16/8

TEST CONCENTRATIONS
Range finding study
- Results used to determine the conditions for the definitive study: 96-h LC50 between 100 and 500 mg/L (no pH adjustment)

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: pH adjustment

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 316 - < 464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no pH adjustment

Results with reference substance (positive control):

- Results with reference substance valid? yes (1987-04-03)
- Mortality: corresponds to normal sensitivity
- LC50: 31 mg/L (48 h)

Sublethal observations / clinical signs:

Symptoms

Nominal concentration [mg/L]	Symptoms observed after
	4h	24h	48h	72h	96h
0 (control)
100
147
215			T
316			A	A
464	L,T		A
464*

* pH adjusted  L: gasping  A: apathy  T: tumbling

Mortality

Nominal concentration [mg/L]	Mortality [%] after
	4h	24h	48h	72h	96h
0 (control)	0	0	0	0	0
100	0	0	0	0	0
147	0	0	0	0	0
215	0	10	20	20	20
316	0	0	0	0	0
464	0	80	100	100	100
464*	0	0	0	0	0

* pH adjusted  

pH-values / oxygen content

Nominal concentration [mg/L]	pH / oxygen content [mg/L]
	0h	24h	48h	72h	96h
0 (control)	8.0 / 8.4	8.4 / 8.0	8.4 / 7.8	8.1 / 8.2	8.3 / 8.1
100	8.7 / 8.3	8.4 / 7.4	8.2 / 7.8	8.0 / 7.8	8.2 / 8.3
147	8.8 / 8.1	8.6 / 7.3	8.2 / 7.1	8.0 / 7.3	8.3 / 7.8
215	9.0 / 8.3	8.8 / 8.1	8.4 / 7.8	8.0 / 8.1	8.2 / 8.3
316	9.1 / 8.3	8.7 / 8.3	8.4 / 7.6	8.1 / 8.0	8.2 / 8.4
464	9.3 / 7.9	9.0 / 8.2	8.8 / 9.1
464*	7.6 / 8.2	8.0 / 8.0	8.0 / 8.3	7.7 / 8.2	8.0 / 8.5

*pH adjusted

Validity criteria fulfilled:

yes

Conclusions:

LC 50 (96 h) values of > 316 - < 464 mg/L (no pH adjustment) and > 464 mg/L (pH adjusted) were determined.

Executive summary:

The acute toxicity of Butyldiethanolamine towards Leucisdus idus was investigated according to German standard DIN 38412 in compliance with GLP (BASF, 1987). Test organisms were exposed to 0 (control), 100, 147, 215, 316, 464 and 464 (pH adjusted) mg/L of the test substance for 96 hours based on nominal test concentrations. The values were not analytically verified, but are assumed to be stable due to the low n-octanol/water partition coefficient (log Kow 0.58), the high water solubility (WS of 10,000 mg/L), the moderate vapor pressure (VP 1.2 Pa at 25 °C) and the low Henry´s Law Constant (HLC 2.04 x E-5 Pa*m³/mol at 25 °C) of the chemical. One replicate with 10 animals for each test concentration and the control was prepared. As positive control, the reference substance chloroacetamide was used. As final result, LC 50 (96 h) values of > 316 - < 464 mg/L (no pH adjustment) and > 464 mg/L (pH adjusted) were determined.Validity criteria for the reference substance were fulfilled.

Description of key information

Butyldiethanolamine_DIN 38412: LC50 (96 h) > 316 mg/L (no pH adjustment)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

316 mg/L

Additional information

The acute toxicity of Butyldiethanolamine towards Leucisdus idus was investigated according to German standard DIN 38412 in compliance with GLP (BASF, 1987). Test organisms were exposed to 0 (control), 100, 147, 215, 316, 464 and 464 (pH adjusted) mg/L of the test substance for 96 hours based on nominal test concentrations. The values were not analytically verified, but are assumed to be stable due to the low n-octanol/water partition coefficient (log Kow 0.58), the high water solubility (WS of 10,000 mg/L), the moderate vapour pressure (VP 1.2 Pa at 25 °C) and the low Henry´s Law Constant (HLC 2.04 x E-5 Pa*m³/mol at 25 °C) of the chemical. One replicate with 10 animals for each test concentration and the control was prepared. As positive control, the reference substance chloroacetamide was used. Validity criteria for the reference substance were fulfilled.

As final result, LC 50 (96 h) values of > 316 - < 464 mg/L (no pH adjustment) and > 464 mg/L (pH adjusted) were determined.