4-bromo-3,3,4,4-tetrafluorobut-1-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 January 1980 - 22 February 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

Although a subacute study is not typically used to determine an acute inhalation classification, this study included concentrations 4 times above the current recommended toxicity testing limit. The exposure concentrations were measured, and rats were treated for 6 hours per day. Since no mortality or significant clinical signs beyond narcosis were noted, this study is considered sufficient to support a STOT SE 3 classification. Pathology examinations and clinical chemistry measurements were also included in the study allowing a reliable prediction of no classification for acute toxicity by inhalation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

- Principle of test: to observe the effects of the test substance - BTFB on male rats after repeated inhalation exposures over a 2 week period.
- Short description of test conditions: Male Crl:CD rats were exposed to BTFB at 0.10% or 1.03% in air with the control group exposed to air only for 6hrs/day, 5 days/week for 2 weeks. Clinical observations were made throughout the testing period with clinical chemical analyses performed following 10th exposure. Pathological examinations performed following necropsy with body and organ weigh analysis performed.
- Parameters analysed / observed:Clinical observations, pathological analysis, clinical chemistry, body weight

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-bromo-3,3,4,4-tetrafluorobut-1-ene

EC Number:

242-440-8

EC Name:

4-bromo-3,3,4,4-tetrafluorobut-1-ene

Cas Number:

18599-22-9

Molecular formula:

C4H3BrF4

IUPAC Name:

4-bromo-3,3,4,4-tetrafluorobut-1-ene

Test material form:

not specified

Test animals

Species:

rat

Strain:

other: Crl:CD

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Remarks:

via glass exposure chambers

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Glass exposure chambers
- Method of holding animals in test chamber: Not stated
- Source and rate of air: Not stated
- Method of conditioning air: Not stated
- System of generating particulates/aerosols: Not stated
- Temperature, humidity, pressure in air chamber: Temperature = less than or equal to 30˚C, Atmosphere = greater than or equal to 19%
- Air flow rate: Not stated
- Air change rate: Not stated
- Method of particle size determination: Not stated
- Treatment of exhaust air: Not stated

TEST ATMOSPHERE
- Brief description of analytical method used: Test item vapours were analysed on a HP 5700A GC with flame ionised detector and an injection splitter.
- Samples taken from breathing zone: yes

VEHICLE (if applicable)
- Justification for use and choice of vehicle: Not stated
- Composition of vehicle: Air
- Type and concentration of dispersant aid (if powder): N/A
- Concentration of test material in vehicle: 0.1% and 1%
- Lot/batch no. of vehicle (if requir

Analytical verification of test atmosphere concentrations:

yes

Remarks:

GC with flame ionisation detector

Duration of exposure:

6 h

Remarks on duration:

5 days a week for 2 weeks

Concentrations:

1% = 84650 mg/m3 = 84.65 mg/L
0.1% = 8465 mg/m3 = 8.465 mg/L

No. of animals per sex per dose:

10

Control animals:

yes

Remarks:

0% concentration

Details on study design:

- Dose selection rationale: Not stated
- Rationale for animal assignment (if not random): Not stated
- Fasting period before blood sampling for clinical biochemistry: Not stated
- Rationale for selecting satellite groups: Not stated
- Post-exposure recovery period in satellite groups: Not stated
- Section schedule rationale (if not random): N/A
- Other: N/A

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC0

Effect level:

84 650 mg/m³ air

Based on:

test mat.

Exp. duration:

6 h

Mortality:

none

Clinical signs:

other: decreased response to sound and loss of coordination.

Body weight:

The mean body weights of animals exposed to test material at 1.0% were significantly lower than those in the control group from exposure 5 through to the recovery period. Rats showed normal rate of weight gain during the recovery period.