Methyltrioctylammonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Methyltrioctylammonium chloride
- Molecular formula: C25H54ClN
- Molecular weight: 404.162 g/mole
- Smiles :[N+](CCCCCCCC)(CCCCCCCC)(CCCCCCCC)C.[ClH-]
- Inchl: 1S/C25H54N.ClH/c1-5-8-11-14-17-20-23-26(4,24-21-18-15-12-9-6-2)25-22-19-16-13-10-7-3;/h5-25H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Viscous liquid (colorless to yellow)

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The stock solution 20 mg/l was prepared by dissolving colourless dense liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample in reconstituted water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

± 1 hr

Test temperature:

20±1°C

pH:

sample at concentration 0.2 mg/l: 8 changes to 7.9
Control 1: 8 changes to 7.9
Control 2: 7.8 changes to 7.9 during test

Dissolved oxygen:

higher than 7.6 mg/L at the end of test

Nominal and measured concentrations:

0, 0, 0.006, 0.012, 0.025, 0.050, 0.100, 0.200 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.031 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CI was 0.018 - 0.052 mg/l

Results with reference substance (positive control):

- Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)

Reported statistics and error estimates:

EC50 was calculated using non linear regression by the software Prism 4.0

Validity criteria fulfilled:

yes

Conclusions:

The median effective concentration (EC50) for the test substance Methyltrioctylammonium chloride, in Daphnia magna was determined to be 0.031 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Executive summary:

Aim of this study was to assess the short term toxicity of Methyltrioctylammonium chloride to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 20 mg/l was prepared by dissolving colourless dense liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0, 0.006, 0.012, 0.025, 0.050, 0.100, 0.200 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance Methyltrioctylammonium chloride, in Daphnia magna was determined to be 0.031 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP classification criteria.

Description of key information

Aim of this study was to assess the short term toxicity of Methyltrioctylammonium chloride to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 20 mg/l was prepared by dissolving colourless dense liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0, 0.006, 0.012, 0.025, 0.050, 0.100, 0.200 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Methyltrioctylammonium chloride, in Daphnia magna was determined to be 0.031 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.031 mg/L

Additional information

Summarized result from experimental reports and peer reviewed journals for the toxicity of test chemical on the mortality of invertebrates were studied and mention as follows:

In the first experimental study from experimental report 2018, Aim of this study was to assess the short term toxicity of Methyltrioctylammonium chloride to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 20 mg/l was prepared by dissolving colourless dense liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample in reconstituted water. 0, 0, 0.006, 0.012, 0.025, 0.050, 0.100, 0.200 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Methyltrioctylammonium chloride, in Daphnia magna was determined to be 0.031 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP classification criteria.

Above experimental study was supported by the study from peer reviewed journal. Aim of the study was to determine the effect of test chemical on the growth and abnormalities of daphnia magna in the presence and absence of acetone. Stock solutions were made either in acetone or in water. For stock solutions in water appropriate amounts of Aliquat 336 and 5 mL deionized water were sonicated in a 10-mL vial for 30 s, diluted by Standard. Reference Water and allowed to equilibrate for one h. Final test concentrations, which were selected by progressive bisectioning on logaritmic scale, were prepared by diluting appropriate volumes of stock solutions in SRW up to 190 mL, and left for equilibration for 2 h. The final I0 mL SRW was then added together with the animals. Less than 66 hrs old daphnia magna were used in the study. 9-14 animals per concentration was added and performed in duplicates or triplicates. The animals were classified as dead when their second antenna did not move within 15 s after irrigation by a water stream from the handling pipet. Dead and live animals were recorded after 3, 24, 48, 72, and 96 h. After the exposure of test chemical with the water flea daphnia magna, the LC50 was determine to be ranges from 0.0316 – 0.0097 mg/l in the absence of acetone. In the presence of acetone the LC50 was ranges from 0.0129 – 0.0157 mg/l.

Similarly in the third study from peer reviewed journal, the study was conducted to access the level of toxic effect of test chemical Methyltrioctylammonium chloride on the mobility of daphnia magna after the exposure period of 48 hrs. Toxicant solutions (200 ml) were prepared by addition of pre-made Stock solutions of the toxicants to SRW (Standard reference water). Stock solutions were made by dissolving weighed amounts of the toxicants in acetone. stock solution was made for each concentration in order to produce desired nominal concentrations on addition of 50 µl stock solution to 200ml water (toxicant dilution factor 4000 x : final acetone conch 250 µl /I). For the stock culture the < 24 hrs old water fleas were kept in a 501 glass tank filled with 401 aged, aerated tap water, the composition of which has been reported previously. Twice a week 1/4 of the volume was changed and approx. 2/3 of the neonates were removed in order to avoid crowding and stagnation of the culture. 300 ml glass beakers filled with 200 ml of stock solution having headspace of 100 ml were used. Mortality was recorded after 3, 24, 48, 72 and 96 hr. Other reactions of the animals like swimming at the surface and general activity (swimming or resting) were also recorded. After the exposure of test chemical Methyltrioctyl -ammonium chloride with daphnia magna for 96 hrs, the LC50 was determine to be 0.031 mg/l on the basis of mortality of daphnia magna. After 24 hrs the LC50 was determine to be 0.088 MG/L. After 48 and 72 hrs the LC50 was determine at 0.052 MG/L and 0.039 MG/L. based on the LC50 value, chemical consider to be toxic and can be consider to be classified as aquatic acute 1 / chronic 1 as per the CLP classification criteria.

Thus based on the overall studies chemical Methyltrioctylammonium chloride (CAS No. 5137 -55 -3), consider to be toxic and can be consider to be classified as aquatic acute 1 / chronic 1 as per the CLP classification criteria.