Methyltrioctylammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Study was conducted for the evaluation of acute effect of test chemical Methyltrioctylammonium chloride on the mortality rate of zebrafish under static system for 144 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Methyltrioctylammonium chloride
- Molecular formula: C25H54ClN
- Molecular weight: 404.162 g/mole
- Smiles :[N+](CCCCCCCC)(CCCCCCCC)(CCCCCCCC) C.[ClH-]
- Inchl: 1S/C25H54N.ClH/c1-5-8-11-14-17-20-23-26(4,24-21-18-15-12-9-6-2)25-22-19-16-13-10-7-3;/h5-25H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Viscous liquid (colorless to yellow)
- Analytical purity: 88 %
- Impurities (identity and concentrations): 12 %

Analytical monitoring:

not required

Vehicle:

yes

Remarks:

Acetone

Details on test solutions:

Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Toxicant solutions were prepared by pipetting into wide mouthed Erlen-Meyer flasks appropriate amounts (0-5.6 ml) of toxicant stock solutions made up in acetone. After evaporation of the acetone just to dryness at room temperature, 1000 ml of premade aerated SRW2 (Standard reference water) was added to each flask. A solvent control (maximal amount of acetone evaporated just to dryness + 1000 ml diluent) and an ordinary control (1000 ml diluent) were included in the entire test procedure. All solutions were allowed to stand over night at the test temperature (25°C).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Details on test organisms
TEST ORGANISM
- Common name: Zebrafish
- Age at study initiation (mean and range, SD): Juvenile
- Weight at study initiation (mean and range, SD): 0.25 g
- Feeding during test: No feeding

Test type:

static

Water media type:

freshwater

Total exposure duration:

144 h

Remarks on exposure duration:

3, 6, 24, 48, 72, 96, 120 and 144 hr

Hardness:

100 mg/I as CaCO3

Test temperature:

25 ° C

pH:

7.3-8.5

Nominal and measured concentrations:

Nominal

Details on test conditions:

Details on test conditions
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- No. of organisms per vessel: 5

Reference substance (positive control):

not specified

Key result

Duration:

144 h

Dose descriptor:

LC50

Effect conc.:

0.137 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI was 0.110-0.171 mg/l. After 96 hrs also mortality were observed at 0.137 mg/l

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.108 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI was 0.100 – 0.135 mg/l

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

0.131 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CI was 0.100-0.180 mg/l

Reported statistics and error estimates:

probit analysis

Validity criteria fulfilled:

not specified

Conclusions:

As the zebrafish exposed with the test chemical Methyltrioctylammonium chloride for 144 hrs, mortality were observed. The effect were measured at 24, 48, 96 and 144 hrs. The LC50 after 96 and 144 hrs observed at 0.137 mg/l. whereas after 24 and 48 hrs LC50 ranges from 0.108 MG/L to 0.131 MG/L.

Executive summary:

Study was conducted for the evaluation of acute effect of test chemicalMethyltrioctylammonium chloride on the mortality rate of zebrafish under static system for 144 hrs. Toxicant solutions were prepared by pipetting into wide mouthed Erlen-Meyer flasks appropriate amounts (0-5.6 ml) of toxicant stock solutions made up in acetone. After evaporation of the acetone just to dryness at room temperature, 1000 ml of premade aerated SRW2 (Standard reference water) was added to each flask. A solvent control (maximal amount of acetone evaporated just to dryness + 1000 ml diluent) and an ordinary control (1000 ml diluent) were included in the entire test procedure. All solutions were allowed to stand over night at the test temperature (25°C).

5 Juvenile zebrafish were used. Effect measured in the interval of 3, 6, 24, 48, 72, 96, 120 and 144 hr. Effect on the mortality were measured and LC50 noted by probit analysis. As the zebrafish exposed with the test chemical Methyltrioctylammonium chloride for 144 hrs, mortality were observed. The effect were measured at 24, 48, 96 and 144 hrs. The LC50 after 96 and 144 hrs observed at 0.137 mg/l. whereas after 24 and 48 hrs LC50 ranges from 0.108 MG/L to 0.131 MG/L. Thus based on the LC50 value, chemical consider to be toxic and can be consider to be classified as aquatic acute 1 / chronic 2 category as per the CLP classification criteria.

Description of key information

Study was conducted for the evaluation of acute effect of test chemicalMethyltrioctylammonium chloride on the mortality rate of zebrafish under static system for 144 hrs. Toxicant solutions were prepared by pipetting into wide mouthed Erlen-Meyer flasks appropriate amounts (0-5.6 ml) of toxicant stock solutions made up in acetone. After evaporation of the acetone just to dryness at room temperature, 1000 ml of premade aerated SRW2 (Standard reference water) was added to each flask. A solvent control (maximal amount of acetone evaporated just to dryness + 1000 ml diluent) and an ordinary control (1000 ml diluent) were included in the entire test procedure. All solutions were allowed to stand over night at the test temperature (25°C).

5 Juvenile zebrafish were used. Effect measured in the interval of 3, 6, 24, 48, 72, 96, 120 and 144 hr. Effect on the mortality were measured and LC50 noted by probit analysis. As the zebrafish exposed with the test chemical Methyltrioctylammonium chloride for 144 hrs, mortality were observed. The effect were measured at 24, 48, 96 and 144 hrs. The LC50 after 96 and 144 hrs observed at 0.137 mg/l. whereas after 24 and 48 hrs LC50 ranges from 0.108 MG/L to 0.131 MG/L. Thus based on the LC50 value, chemical consider to be toxic and can be consider to be classified as aquatic acute 1 / chronic 2 category as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.137 mg/L

Additional information

Summarized result from peer reviewed journals for the toxicity of test chemical on the mortality and embryo development of fishes was studied and mention as follows:

In the first study from peer reviewed journal, Study was conducted for the evaluation of acute effect of test chemicalMethyltrioctylammonium chloride on the mortality rate of zebrafish under static system for 144 hrs. Toxicant solutions were prepared by pipetting into wide mouthed Erlen-Meyer flasks appropriate amounts (0-5.6 ml) of toxicant stock solutions made up in acetone. After evaporation of the acetone just to dryness at room temperature, 1000 ml of premade aerated SRW2 (Standard reference water) was added to each flask. A solvent control (maximal amount of acetone evaporated just to dryness + 1000 ml diluent) and an ordinary control (1000 ml diluent) were included in the entire test procedure. All solutions were allowed to stand over night at the test temperature (25°C).

5 Juvenile zebrafish were used. Effect measured in the interval of 3, 6, 24, 48, 72, 96, 120 and 144 hr. Effect on the mortality were measured and LC50 noted by probit analysis. As the zebrafish exposed with the test chemical Methyltrioctylammonium chloride for 144 hrs, mortality were observed. The effect were measured at 24, 48, 96 and 144 hrs. The LC50 after 96 and 144 hrs observed at 0.137 mg/l. whereas after 24 and 48 hrs LC50 ranges from 0.108 MG/L to 0.131 MG/L. Thus based on the LC50 value, chemical consider to be toxic and can be consider to be classified as aquatic acute 1 / chronic 2 category as per the CLP classification criteria.

First study was supported by the second from peer reviewed journal 1981. Embryo-larval toxicity by the test chemical Methyltrioctylammonium chloride to zebrafish was studied under the flow-through system. Chemical exposed for 10 days with the zebrafish. Stock solutions of test chemical were made up with acetone. Appropriate volumes of stock solution were then pipetted into the dishes and evaporated to dryness at room temperature. Control dishes were prepared in each test series by evaporating the greatest volume of acetone used in the corresponding series. The dilution water (50 ml) was added to the dishes. Test animal not fed during the test. But before test Broodstock fish were fed once with dry food (Tetramin) and once with live water fleas each day, except for the weekends when only dry food was given. After each breeding the fishes were returned to the stock aquaria, in order to keep the sexes separated from each other. The eggs were collected approx 5 hrs after fertilization, which corresponds to development stage 13-14. They were rinsed from fecal matters and viable eggs were transferred to Petri-dishes containing toxicant in dilution water. In each petri-dish 10 – 15 viable eggs/dish added. Eggs were considered dead as they turned opaque. Hatched larvae were considered dead when no response to mechanical stimulation occurred. The survival time recorded for each egg or larva is identical to the exposure time when each individual was found dead in connection with the countings. After the exposure of test chemical Methyltrioctylammonium chloride for 10 days with test organism zebrafish effect on the embryos were observed. The LC50 was determine at 0.028 MG/L which was calculated by probit analysis. Thus based on the LC50 value, chemical consider to be toxic and can be consider to be classified as aquatic acute 1/ chronic 1 category as per the CLP classification criteria.

Short term acute toxicity of fish rainbow trout, Salmo guirdneri was evaluated for test material Methyltrioctylammonium chloride in static condition for 120 h. Local tap water was used for the test . At each concentration of test material ten fry were exposed in a volume of 2 dm-3 for at least 120 h. Median lethal concentration (LC50) was calculated by graphic estimation on log-probit paper. The LC50 value were noted at 24,48,96 and 120 h. The medial lethal effect concentration (LC50) was observed to be 0.050 mg/l when observed for 120 h .

On the basis of above effect concentration it can be concluded the test material is highly toxic to fish and hence, can be classified as aquatic chronic 1 as per CLP criteria.

Thus based on the above studies, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP criteria.