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EC number: 202-851-5 | CAS number: 100-42-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Styrene
- EC Number:
- 202-851-5
- EC Name:
- Styrene
- Cas Number:
- 100-42-5
- Molecular formula:
- C8H8
- IUPAC Name:
- ethenylbenzene
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Styrene
- Physical state: liquid
- Analytical purity: 99.86 % (w/w)
- Impurities (identity and concentrations): ethylbenzene 0.040 % (w/w), α-methylstyrene 0.030 % (w/w)
- Lot/batch No.: 20041209
- Stability under test conditions: Polymerized in air, light, heat and peroxyacid. Polymerization inhibitor is added (approx. 20 ppm). Test substance was stable during the dosing period.
- Storage condition of test material: cold and dark (refrigerator)
- Other: Supplier: Asahi Kasei Chemicals Corporation
Test animals
- Species:
- rat
- Strain:
- other: Crj: CD (SD) IGS
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Japan (Hino Breeding Center)
- Age at study initiation: males 7 wks, females 8 wks
- Weight at study initiation: males 252.3 - 277.3 g, females 217.9 - 233.6 g
- Fasting period before study:
- Housing: individually in stainless steel cages with wire mesh floors in a barrier-system animal room
- Diet (e.g. ad libitum): autoclaved MF pelleted diet (lot no. 041102, Oriental Yeast) ad libitum
- Water (e.g. ad libitum): clorinated water from the Hita City supply ad libitum
- Acclimation period:1 wk
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.6 - 23.9
- Humidity (%): 51.8 - 56.6
- Air changes (per hr): 10 - 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: clipped dorsal area of the trunk (5x4 cm)
- % coverage: approx. 10 % of the total body surface area
- Type of wrap if used: test site covered with a cotton lint (4x4 cm, Nankei Sangyo) and adhesive bandage (ALCARE)
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, with Otsuka distilled water, lot no. 3G79N, Otsuka Pharmaceutical Factory
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2.21 ml/kg
- Concentration (if solution): undiluted
- Constant volume or concentration used: yes - Duration of exposure:
- 24 h
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- other: yes, sham-exposed
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical signs were recorded just after dosing and 5, 10 and 30 min and 1, 2, 3 and 4 hr after dosing, twice daily from day 1 to 3 after dosing, and thereafter once daily by day 14. Body weights were measured just before dosing and on days 1, 7 and 14 post-dosing.
- Necropsy of survivors performed: yes, all animals were euthanized at the termination of the observation period by exsanguination under ether anesthesia, and subjected to a detailed gross necropsy.
- Other examinations performed: clinical signs, body weight - Statistics:
- The mean and standard deviation in each group were calculated in the body weights.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Remarks on result:
- other: Limit test
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- 2 000 mg/kg bw
- Remarks on result:
- other: Limit test
- Mortality:
- No deaths occurred in males and females.
- Clinical signs:
- other: Control: Decreased spontaneous locomotion was observed in the control group from 30 min to 4 hr (males) and 1 hr to 4 hr (females) after dosing, and disappeared on day 1. 2000 mg/kg: Decreased spontaneous locomotion from 5 min to 4 hr after dosing in 5/
- Gross pathology:
- No abnormalities were observed.
Any other information on results incl. tables
Summary of body weights (mean ± S.D.):
|
Male |
Female |
||
Observation period (day) |
Control |
2000 mg/kg |
Control |
2000 mg/kg |
0 |
261.3 ± 10.0 |
265.0 ± 5.4 |
222.9 ± 4.3 |
227.4 ± 4.7 |
1 |
262.1 ± 11.9 |
268.0 ± 7.5 |
220.4 ± 8.6 |
216.0 ± 9.6 |
7 |
318.3 ± 20.9 |
319.0 ± 11.8 |
254.6 ± 16.5 |
251.6 ± 6.7 |
14 |
368.3 ± 26.2 |
363.4 ± 20.3 |
274.5 ± 19.3 |
274.7 ± 11.7 |
Decreased spontaneous locomotion was considered to be not test substance-related, since the same sign was also noted in the control group and considered due to compression by the adhesive bandage. Incomplete eyelid opening, writhing and vocalization were considered related to irritation, as the test substance is classified as eye and skin irritant in the List of Dangerous Substances in EU (JETOC: No. 97; ECETOC: Technical Report No. 66), however, all of them disappeared on the next day after dosing. Decreased body weights observed in females were considered due to compression of the bandage, as it occurred in the control group, as well.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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