Phenetole

Endpoint summary

Additional information

Short-term toxicity to fish

Based on predicted data of target substance and studies of readacross reviewed for short term toxicity to fish  from reliable sources having Klimisch rating 2 and 4 considering weight of evidence approach. The summary of the results are presented below

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1.	LC50	103.4304 mg/l	mortality	96 hrs.	Lepomis macrochirus	Predicted data
2.	LC50	100.5091mg/l	mortality	96 hrs.	Pimephales promelas	Predicted data
3.	LC 50	32.95 mg/l	mortality	96 hrs.	Pimephales promelas	DEPA
3.	LC50	120 mg/l	mortality	48 hrs	Leuciscus idus melanotus	Publication(RA-100-66-3)
4.	LC50	344 mg/l	mortality	96 hrs	Pimephales promelas	Publication(RA-122-99-6)
5.	LC50	165 mg/l 135 mg/l	mortality	24 hrs 48 hrs	Ptychocheilus oregonensis	Publication(RA-122-99-6)

 

By applying weight of evidence approach the endpoint values varies between 100.5 to 344 mg/l in a 24 to 96 hrs.study on the basis of mortality effect.The LC 50 value 32.95 mg/L is a classifying value.However the data is insufficient for classifying the substance. Thus, considering the CLP Criteria for aquatic classification of the substance and weight of evidence approach , it is concluded that Phenetole does not exhibit short term toxicity to fish (Lepomis macrochirus, Pimephales promelas, Leuciscus idus melanotus & Ptychocheilus oregonensis).

 

Long-term toxicity to fish

According to column I of annex IX, Long-term toxicity testing on fish was considered for waiver as short term toxicity data has already been provided as part of Annex VIII requirements.

Short-term toxicity to aquatic invertebrates

Based on predicted data of target substance(K2) and studies of readacross reviewed for short term toxicity toaquatic invertebrate from reliable sources having Klimisch rating 4 considering weight of evidence approach. The summary of the results are presented below

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1.	EC50	117.0316 mg/L	Intoxication	48 hrs	Daphnia magna	Predicted data
2.	LC50	488 mg/L	Mortality	48 hrs	Daphnia magna	Study report(RA-122-99-6)
3.	LC50	2.7 mg/L	Mortality	48 hrs	Daphnia magna	DEPA

 

By applying weight of evidence approach the endpoint values for target was assessed as follows- EC50-117.0316 mg/L,LC 50 value of target was predicted to be 2.7 mg/l. However the data was insufficient to classify the substance in aquatic chronic category. Thus considering the weight of evidence approach for target and readacross values it was concluded that phenetole does not exhibit toxicity toaquatic invertebrate.

Long-term toxicity to aquatic invertebrates

According to column 1 of Annex IX;Long-term toxicity testing on invertebrate was considered for waiver since the information on acute toxicity has already been provided as a part of Annex VII requirements.

Toxicity to aquatic algae and cyanobacteria

Based on studies reviewed for toxicity to aquatic algae and cyanobacteria from reliable sources having Klimish rating 2and 4 for target and readacross.

The summary of the results are presented below

Sr.no	Endpoint	Effect values	Basis for effects	duration	species	Remarks
1	EC50	162mg/l	Growth	96 hrs.	Pseudokirchnerella subcapitata	Publication RA 100 -66 -3
2	NOEC	3.211644 mg/l	Growth	72 hrs.	Pseudokirchnerella subcapitata	Predicted data
3	LOEC	21.7231 mg/l	Population	120 hrs.	Pseudokirchnerella subcapitata	Predicted data

 

By applying weight of evidence approach the endpoint values was found to be EC50=162mg/l;NOEC=3.211644mg/l and LOEC=21.7231 mg/l in a 72 to 120 hrs.study on the basis of growth and population effect.Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that Phenetole does not exhibit toxicity to aquatic algae and cyanobacteria(Pseudokirchnerella subcapitata).

Toxicity to microorganisms

Based on the various studies reviewed for target substance phenetole for the endpoint toxicity to microorganism with Klimisch rating 2 and 4.The results are summarized as follows-

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1.	EC50	7.3 mg/L	Growth inhibition	30 min	Photobacterium phosphoreum	Publication
2.	IGC50	169 mg/L	Growth inhibition	48 hrs	Tetrahymena pyriformis	Publication
3.	pT	5.33 mg/L	Physiology	4.98 min	Vibrio fisheri	Publication

Based on the above values the inhibition concentration varies from 5.33 -169 mg/L.Also the EC50 value was assessed to be7.3 mg/L.