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Description of key information

Acute Oral LD50 > 2000 mg/kg
Acute Dermal L50 > 2000 mg/kg

Key value for chemical safety assessment

Additional information

The acute oral toxicity of C4-Acrylate in male and female Wistar rats was assessed using the Acute Toxic Class Method. The test article was administered by oral gavage to the three Wistar rats of each sex at 2000 mg//kg body weight. Animals were subjected to daily observations and weekly determination of body weight. Macroscopic examination was performed after terminal sacrifice. No mortality or abnormal macroscopic findings after necropsy were observed and the body weight gain shown by the animals was considered normal. Lethargy, hunched posture, uncoordinated movements and/or chromodacyorrhoea was observed in females between days 1 and 3. Lethargy in all males between days 1 and 2, and hunched posture was shown by one male on days 1 and 2. The oral LD50 of the test article was established to exceed 2000 mg/kg body weight. Based on these results and according to EC criteria for classification and labelling requirements, C4 Acrylate does not have to be classified and has no obligatory labelling requirements for oral toxicity.

 

The acute dermal toxicity of C4-Acrylate (off-white to white waxy solid, composition 93.96 w%, Batch 40177) was evaluated in Wistar rats. The study was performed in compliance with OECD GLP (1997). The study design was based on OECD No 402 (1987), EC 440/2008 B3 L142 (2008), EPA OPPTS 870.1200 (1998), and JMAFF No. 8147 (2011). The test article was prepared in polyethylene glycol (w/w) and dermally applied to a 5 x 7 cm area of clipped fur on male and female rats (5/sex) for a single application at a 2000 mg/kg body weight (10 mL/kg) dose. The application represented 10% total body surface of the rats and was applied with a gauze patch backing and covered in aluminum foil, an elastic bandage, and tape (females only). After 24 hours, the dressing was removed and the skin cleaned of any residual test substance with tap water. Observations were made for mortality (twice daily), body weights (Day 1, 8, and 15), and clinical signs of toxicity (Days 1-15). All animals were euthanized at the end of Day 15 and a necropsy was performed. All animals survived for the length of the study and no abnormalities were found at time of necropsy. Hunched posture (1 male), chromodarcryorrhoea (2 males and 3 females), and pilioerection (1 female) were noted only on Day 1. Body weights were within a historical expected range for the duration of the study. Based on the results of the study, the dermal LD50 of C4 Acrylate is greater than 2000 mg/kg body weight.

Justification for classification or non-classification

The test results for acute dermal and oral toxicity tests do not meet the criteria for classification.