3,3'-dioctadecyl-1,1'-methylenebis(4,1-phenylene)diurea

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

January 2001

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

August 2001

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: 7ET2090
- Expiration date of the lot/batch: 31 December 2007
- Purity: 99 %

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature in the dark
- Stability under test conditions: Stable

Radiolabelling:

no

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Alliance Separation Module 2695 (Waters, Milford, MA, USA)
- Type, material and dimension of analytical (guard) column: Hypersil BDS-CN; 150 mm x 4.6 mm i.d., dp = 5 μm (Alltech, Deerfield, IL, USA)
- Detection system: Dual Absorbance Detector 2487 (Waters)

MOBILE PHASES
- Type: 55/45 (v/v) methanol/Milli-Q water

REFERENCE SUBSTANCES
- Identity: 2,4-DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10.5 mg/L of test substance was dissolved in tetrahydofuran. The solution was ultrasonicated for 15 minutes.
- Quantity of reference substances: A stock solution of the reference substance was prepared in methanol to achieve a concentration of 0.98 g/L. This solution was 100-fold diluted with 55/45 (v/v) methanol/Milli-Q water.

REPETITIONS
- Number of determinations: Reference substances and test item were injected in duplicate, blank solutions were analysed by single injection

EVALUATION
- Determination of the log Koc value: The retention time of the reference substance 2,4-DDT (log Koc = 5.63) was <10 minutes. The retention time of the test substance was >10 minutes. When compared to the reference substance the log Koc value of the test substance was determined to be >5.63 (Koc > 427000).

Key result

Sample No.:

#1

Type:

log Koc

Value:

> 5.63 dimensionless

Key result

Sample No.:

#2

Type:

Koc

Value:

427 000 dimensionless

Validity criteria fulfilled:

not specified

Conclusions:

The Koc and log Koc values of the test item were 427000 and >5.63, respectively.

Executive summary:

Oudhoff (2007) is a GLP-compliant study following OECD guideline 121, HPLC method. The study is reliable without restrictions (Klimisch 1) as it followed a standard method. The reference substance 2,4 -DDT had a log Koc of 5.63. When compared to the reference substance the log Koc and Koc values of the test item were 427000 and >5.63, respectively.

Description of key information

The Koc and log Koc values of the test item were 427000 and >5.63, respectively.

Key value for chemical safety assessment

Koc at 20 °C:

427 000

Additional information

Oudhoff (2007) is a GLP-compliant study following OECD guideline 121, HPLC method. The study is reliable without restrictions (Klimisch 1) as it followed a standard method. The reference substance 2,4 -DDT had a log Koc of 5.63. When compared to the reference substance the log Koc and Koc values of the test item were 427000 and >5.63, respectively.