A mixture of: 3,3'-dicyclohexyl-1,1'-methylenebis(4,1-phenylene)diurea; 3-cyclohexyl-1-(4-(4-(3-octadecylureido)benzyl)phenyl)urea; 3,3'-dioctadecyl-1,1'-methylenebis(4,1-phenylene)diurea

Endpoint summary

Description of key information

Table: Overview of aquatic toxicity key information

Endpoint	Species	Test guideline / GLP	Effect value	Comments
Fish acute toxicity	D. rerio	OECD TG 203 / GLP	96-h LC50: > limit of solubility in test medium	At a nominal loading rate of 1000 mg/L*. No test substance analysis was performed due to the low water solubility of the test item.
Aquatic invertebrates acute toxicity	D. magna	OECD TG 202 / GLP	24-h EC50: > limit of solubility in test medium	At a nominal loading rate of 1000 mg/L*. No test substance analysis was performed due to the low water solubility of the test item.
Algae toxicity	P. subcapitata D. subspicatus	OECD TG 201 / GLP	72-h ErC50/ErC10: > limit of solubility in test medium	At a loading rate of 100 mg/L*. Recovery of nominal concentrations was within ±20% of initial nominal concentrations.
Aquatic microorganisms toxicity	Activated sludge	OECD TG 209 / GLP	3-h EC50: >1000 mg/L

* Unfiltered suspension of the test item above the water solubility limit

 

Additional note:

The 24-h EC50 value as determined in the Daphnia acute toxicity test is in line with the OECD TG 202 version 1984. Although at present a 48-h EC50 value would be required for assessment, it was decided that no new study (acute or chronic) will be performed due to the absence of a suitable analytical method (see expert statement on hydrolysis). Furthermore, chronic effects are not expected to occur due the limited bioavailability and potential for bioaccumulation of the substance and the complete absence of effects in the available aquatic toxicity dataset. Altogether, the currently available information is considered to be adequate for assessment.