O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to OECD Guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

EC Number:

255-255-2

EC Name:

O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate

Cas Number:

41198-08-7

Molecular formula:

C11H15BrClO3PS

IUPAC Name:

4-bromo-2-chlorophenyl ethyl (propylsulfanyl)phosphonate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Details on preparation and application of test substrate:

- Method of mixing into soil: Stock solution was mixed with 100 g of fine sand. The hexane solvent was evaporated overnight at room temperature. The dry stock mixtures were then mixed with dry premixed artificial soil in a Hobart blender. Water was then added to the blend. - Method of application to soil surface (if used):- Controls: Water and vehicle controls performed- Chemical name of vehicle: Hexane- Evaporation of vehicle before use: Yes.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM- Common name: Earthworm- Source: De T. Wilde, Farm Research, D-7867 Wehr/Baden, Germany- Age at test initiation: > 2 months - Weight at test initiation: 278-370 mgACCLIMATION- Acclimation period: 24 hr- Acclimation conditions: 24 hr conditioning in soil used for the test

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Total exposure duration:

14 d

Test conditions

Test temperature:

22°C ± 2 °C

pH:

5.5-6.2

Moisture:

24-38%

Details on test conditions:

TEST SYSTEM- Test container: Beakers of 1L filled with 750g soil (wet weight), covered with watch glasses- No. of organisms per container (treatment): 10- No. of replicates per treatment group:4- No. of replicates per control: 4- No. of replicates per vehicle control: 4OTHER TEST CONDITIONS- Photoperiod: Continuous illumination - Light intensity: 400/800 Lux cold white fluorescent light.VEHICLE CONTROL PERFORMED: yes

Nominal and measured concentrations:

Nominal concentrations:0, 12.3, 37.0, 111, 333 and 1000 mg/kg dry weight soil.

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

372 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL 298-463 mg/kg dw

Any other information on results incl. tables

Details on mortality levels observed are given in the table below.

Nominal concentration (mg/kg dry weight of soil)	Dead organisms after (cumulated data n=40)
	7 days	14 days	% after 14 days
Blank	0	0	-
Vehicle	0	0	-
12.3	0	0	-
37.0	0	1	2.5
111	0	5	12.5
333	0	12	30
1000	0	38	95

The average weights of worms at the start and the end of the exposure period are given in the following table.

Nominal concentration (mg/kg dry weight of soil)	Average live weight of test organisms (mg)
	Start of exposure	End of exposure
Blank	278	271
Vehicle	370	244
12.3	297	232
37.0	288	216
111	310	241
333	305	194
1000	293	190

Applicant's summary and conclusion

Validity criteria fulfilled:

yes