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Short-term toxicity to aquatic invertebrates

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Description of key information

No immobilisation or other negative effects were observed in any of the daphnids exposed to the test substances up to the highest attainable concentration tested. 

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Additional information

No data are available on the toxicity of (Z)-N-Octadecyldocos-13-enamide (CAS No. 10094-45-8) towards aquatic invertebrates. Therefore data from the analogue substance (Z)-N-octadec-9-enylhexadecan-1-amide (oleyl palmitamide, CAS 16260-09-6) were used as read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5 and justified in detail in the overall summary (IUCLID Chapter 6.1) and within the analogue justification attached in IUCLID Section 13.

A study was performed to assess the acute toxicity of (Z)-N-octadec-9-enylhexadecan-1-amide (oleyl palmitamide, CAS 16260-09-6) to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008. The test substance is highly insoluble in water and pre-study solubility work conducted indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation e. g. ultrasonication and high-shear mixing. A pre-study media preparation trial indicated that the use of a saturated solution method of preparation was most appropriate for this test item. The 48-Hour EC50for the test item to Daphnia magna based on nominal test concentrations was greater than 100% v/v saturated solution. The No Observed Effect Concentration was 100% v/v saturated solution. Chemical analysis of the test preparations at 0 and 48 hours showed measured test concentrations of less than the limit of quantitation (LOQ) of the analytical method employed were obtained which was determined to be 0.0141 mg/L.