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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 2008-09-11 to 2008-11-12
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study, OECD guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
2007-01-12

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name: Lipacide UG
- Batch n°: 712300001
- Appearence: white to pink-fawn powder
- Purity: 97.4%
- Stability: H2O pH 7 2.5g/L: 96h
- Solubility: H2O, EtOH > 1 g/L, acetone < 0.1 g/L, CH3CN, DMSO unknown
- Expiration date: 2010-05-02
- Storage: room temperature in the dark
- Hazard information: Xi irritant
R-phrases: R41: risk of serious damage to eyes
S-phrases: S26: in case of contact with eyes, rinse immediatly with plenty of xater and seek medical advice
S39: wear eye/face protection

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
As solubility surpasses 100mg/L, a solution containing 100 mg/L in dilution water was prepared. The resulting solution was clear and homogeneous. The pH of the solution was determined as 6.5.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Variety STRAUS
Strain: Berlin
Sex: female
Age: between 0 end 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: September, 27th 2007
Selection of the test system was made following the proposal of the guidelines.
Food: unicellular green algae, not during the test
Medium renewal: twice a week
Photo period: 16/8 hours, using neon tubes
T: 20 +/- 2°C

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.50 mmol/L
Test temperature:
23.5 - 24.1 °C
pH:
from 7.2 to 7.7
Dissolved oxygen:
above 8.3 mg/L
Details on test conditions:
- Vessels: glass beakers, nominal volume 50 mL, tall shape
- Replicates: four vessels, each containing 20 mL test solution and five daphnia
- Control: four vessels, each containing 20 mL dilution water and five daphnia
- Feeding: none
- lighting: none
-Temperature: 20 +/-2°C
Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
24h EC50 of K2Cr2O7 was determined at 0.79mg/L
Immobilisation in the controls was 10% at he most
The lowest concentration of dissolved oxygen at the end of the test was 8.4mg/L
The highest pH variation was 0.7 units.
The study can be considered valid
Results with reference substance (positive control):
24h EC50 of K2Cr2O7 was determined at 0.79 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
24h NOEC= 100mg/L
48h NOEC= 100mg/L
24h EC50i> 100mg/L
48h EC50> 100 mg/L
Executive summary:

This study was performed in order to evaluate the toxic potential of Lipacide UG towards freshwater shrimp, using species Daphnia magna. D magna STRAUSS, which belongs to the family of crustacea, was chosen in the guideline as a typical part of zoo-plancton.

Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

No animals were immobilised in the control. 10% of the animals were immobilised in the treatment, which was stated as not significant.

The 24h-EC50i of potassium dichromate was tested in a current refrence test. the value was determined at 0.79mg/L, lying within the demanded range of 0.6 -2.1 mg/L.

at the start and at the end of the test, the content of the test item in the test solutions was determined. the recovery after 48 hours was 97% of the start concentration, the correlation between nominal and measured concentration was very good. Therefore, the determination of the biological results was based on the nominal concentration.

24h NOEC= 100mg/L

48h NOEC= 100mg/L

24h EC50i> 100mg/L

48h EC50i> 100 mg/L