3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 8th to 12th,1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

December 1992

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

July 17th, 1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

A limit test was performed in accordance with the test guidelines to demonstrate that the test substance has no toxic effect on the test fish up to the concentration of nominal 200 mg/l. Thus, the only concentration tested was nominal 200 mg/l and a control (test water without addition of the test substance). The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 96 % to 98 % of the nominal value. Therefore, all reported biological results are related to the nominal concentration of the test substance. Medium renewal was performed every 24 hours to keep the concentrations of test item in the test media as constant as possible during the test period.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

7 fishes in control
7 fishes in the test substance concentration

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

130 mg CaCO3/l

Test temperature:

The test media temperatures ranged from 15.4 to 16.5 °C.

pH:

The measured pH-values in the test media ranged from pH 7.5 to pH 8.6.

Dissolved oxygen:

>= 7.8 mg/l

Nominal and measured concentrations:

Nominal concentration of the test substance = 200 mg/l.
The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 96 % to 98 % of the nominal value.
Therefore, all reported biological results are related to the nominal concentration of the test substance.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: LLC

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

In the control and in the 200 mg/l test substance concentration all fish survived until the end of the test. The test medium with 200 mg/l of test substance was intensively coloured by the test substance. Therefore no observation of test fishese in the test medium was possible. At the end of the test, the test fish were placed in control water for observation of symptoms of intoxication. No symptoms of intoxication were observed. Therefore, the 96-hour NOEC, and the 96-hour LC0 of the test substance to Rainbow trout were determined to be at least 200 mg/l test substance. The NOEC might even be higher than this concentration, but concentrations in excess of nominal 200 mg/l test substance have not been tested. The 96-hour LOEC, the 96-hour LC0, the 96-hour LC50 and the 96-hour LC100 were clearly higher than 200 mg/l test substance. These values could not be quantifred, since concentrations in excess of 200 mg/l have not been tested.

Conclusions:

The test substance has no toxic effects on the fish up to 200 mg/l concentration.

Executive summary:

The short-term acute toxicity to fish species was carried out according OECD test guideline 203 and EU Method C.1 (Acute Toxicity for Fish).

The LC50 estimation for a 96-hour exposure of the test substance is 200 mg/L.

Description of key information

The test substance has no toxic effects on fish up to 200 mg/l concentration.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

200 mg/L

Additional information

The short-term acute toxicity to fish species was carried out according OECD test guideline 203 and EU Method C.1 (Acute Toxicity for Fish).

The LC50 estimation for a 96-hour exposure of the test substance is 200 mg/L.