3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

Hydrolysis

Administrative data

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From August 14th to 20th,1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Version / remarks:

December 1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Version / remarks:

May 12th, 1981

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

EC Number:

424-310-4

EC Name:

3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

Cas Number:

178452-66-9

Molecular formula:

Hill formula: C63 H83 N27 O17 S4 CAS formula: C60 H77 N27 O14 S4 . C3 H6 O3

IUPAC Name:

2-hydroxypropanoic acid; 5-({4,6-bis[(2-aminopropyl)amino]-1,3,5-triazin-2-yl}amino)-3-{2-[4-({4-[(4-{2-[8-({4,6-bis[(2-aminopropyl)amino]-1,3,5-triazin-2-yl}amino)-1-hydroxy-3,6-disulfonaphthalen-2-yl]diazen-1-yl}phenyl)amino]-6-{[3-(diethylamino)propyl]amino}-1,3,5-triazin-2-yl}amino)phenyl]diazen-1-yl}-4-hydroxynaphthalene-2,7-disulfonic acid

Test material form:

solid

Radiolabelling:

no

Study design

Analytical monitoring:

yes

Details on test conditions:

A preliminary test was performed at 50 ± 0.1 °Cat each pH of 4, 7, 9.
Aliquots of each test solution were analysed in time intervals using the HPLC-UV analytical method. Analsyis was perfomed before incubation and after 5 days incubation.

Duration of test

Duration:

5 d

pH:

4

Temp.:

50 °C

Initial conc. measured:

129 - 137 mg/L

Remarks:

deion. water

Number of replicates:

Duplicate for each pH

Results and discussion

Preliminary study:

Test solutions of the test substance dissolved in buffer solutions adjusted to pH 4.0, pH 7.0 and pH 9.0 were found to precipitate, due to agglomeration of the test article with the salts of the buffer solutions.
The concentrations of the half-saturated test solutions containing 1 % dimethylsulfoxide were determined to be lower than 1 mg/l by photometric measurements.
For technical reasons the performance of the hydrolysis study with the test-substance half-saturated in aqueous buffer solutions containing 1 % dimethylsulfoxide is not possible according to the guideline in force. Therefore, the hydrolysis reaction of the test substance was determined in deionized water.
The HPLC chromatograms of the test solutions obtained after an incubation of 5 days at 50.0 °C were compared with the HPLC chromatograms obtained for the untreated test solutions. No additional peaks were found after the performance of the hydrolysis test.
According to this result, it could be stated that a test solution of the test substance in deionised water is hydrolytically stable. According to the guidelines, the criteria of less than 10 % hydrolysis reaction after 5 days was met and therefore no further testing is necessary.
In conclusion, the half-life time of the test substance dissolved in deionised water was estimated to be longer than one year at 25 °C.

Transformation products:

no

Dissipation DT50 of parent compound

pH:

4

Temp.:

25 °C

DT50:

> 1 yr

Details on results:

According to the guidelines, the criteria of less than 10 % hydrolysis reaction after 5 days was met and therefore no further testing is necessary. In conclusion, the half-life time of BLUE PE 3811 dissolved in deionised water was estimated to be longer than one year at 25 °C.

Applicant's summary and conclusion

Conclusions:

The test substance is hydrolytically stable in deionized water.