3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 9th to 11th, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

December 29th, 1992

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Aprile 4th, 1984

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The nominal concentrations tested were 9, 19, 41, 91 and 200 mg /l test substance. Additionally, a control group (test water without addition of the test substance) was tested in parallel. The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 95 % to 105 % of the nominal value. In the test medium the test substance was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration of the test substance.

Test organisms (species):

Daphnia magna

Details on test organisms:

20 daphnids tested per control
20 daphnids tested per each test concentration

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg CaCO3/l

Test temperature:

The test media temperatures ranged from 20.4 to 20.8 °C.

pH:

The measured pH-values in the test media ranged from pH 7.1 to pH 7.9.

Dissolved oxygen:

>= 8.3 mg/l

Nominal and measured concentrations:

Nominal concentrations of the test substance = 9, 19, 41, 91, 200 mg/l.
The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 95 % to 105 % of the nominal value. In the test medium the test substance was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration of the test substance.

Details on test conditions:

In Milli-Q-water salts were added: CaCl2 × 2H2O (2.0 mmo/L = 294.0 mg/l), MgSO4 × 7H2O (0.5 mmol/L = 123.0 mg/l), NaHCO3 (0.75 mmol/l = 65.0 mg/l), KCI (0.75 mmol/l = 5.8 mg/l). Water was aerated to oxygen saturation.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In the control and up to and including the test concentration of 200 mg/l of the test substance no significant rate of immobility or mortality of the test animals was observed during the test period of 48 hours. The immobilization rate of 10 % in the control does not have any influence on the validity of the test because according to the test guidelines this immobilization rate is tolerated in the control and additionally, no other signs of intoxication were observed. In all the test concentrations the test animals were coloured by the test substance. However, this colouration of the Daphnids had no detrimental impact on the survival rate or mobility of the test animals. Therefore, the 48-hour NOEC and the 48-hour EC 0 of Blue PE 3811 to Daphnia magna were determined to be at least 200 mg/l of the test substance. The NOEC might even be higher than this concentration. The 48-hour LOEC, the 48-hour EC 50 and the 48-hour EC 100 were clearly higher than this test concentration. These values could not be quantified, since concentrations in excess of nominal 200 mg/l of the test substance have not been tested. All test media were coloured by the test substance. After 48 hour test duration a small part of the test substance had settled on the bottom ofthe glass beaker and an adsorption of the test substance onto the walls of the test beakers was observed.

Conclusions:

The test substance has no toxic effects on the daphnids up to 200 mg/l concentration.

Executive summary:

The short-term acute toxicity to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

NOEC (48 h) = 200 mg/L

LOEC (48 h) > 200 mg/L

EC50 (48h) >200 mg/L

Description of key information

The test substance hasn't toxic effects on daphnids up to 200 mg/l concentration.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 200 mg/L

Additional information

The short-term acute toxicity to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

NOEC (48 h) = 200 mg/L

LOEC (48 h) > 200 mg/L

EC50 (48h) >200 mg/L