1H-Perylo[3,4-cd]pyridine-1,3(2H)-dione

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study; no deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione

EC Number:

608-929-9

Cas Number:

33955-44-1

Molecular formula:

C22H11NO2

IUPAC Name:

1H-benzo[10,5]anthra[2,1,9-def]isoquinoline-1,3(2H)-dione

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Perylmomoimid Pulver
- Molecular weight: 321,33 g/mol
- Physical state: solid
- Analytical purity: (see Report of the Analytical Laboratory ZAX No: 00L00011)
- Lot/batch No.: Partie 6/99
- Expiration date of the lot/batch: no details provided
- Stability under test conditions:
- Storage condition of test material: no details provided
- Water solubility: 0.31 mg/L at pH 11.3 and 20 °C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4 medium for about 20 hours at 20± 2 0C. Undissolved test substance was removed by centrifugation The nominal concentration of the eluate was 100 mg/L.
By diluting this eluate with M4 medium the following nominal concentrations were prepared: 0, 100, 50, 25, 12.5 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain & Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche
Chimique Appiiquäe, France, in 1978. From this date on this clone was cultured and bred continuously in the
Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Method of breeding:
- Feeding during test: none


ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.41 mmol/L

Test temperature:

20.2 - 20.4 °C

pH:

8.1 - 9.0

Dissolved oxygen:

7.9 - 8.6 mg/L

Nominal and measured concentrations:

Nominal: 0, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Type (delete if not applicable): open
- Fill volume: 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16:8 h day night regime
- Light intensity: About 2 - 7 µE/(m2*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility after 0, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility