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EC number: 608-929-9 | CAS number: 33955-44-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study; data were taken from a structural similar substance (CAS 81-33-4)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Perylene-3,4:9,10-tetracarboxydiimide
- EC Number:
- 201-344-6
- EC Name:
- Perylene-3,4:9,10-tetracarboxydiimide
- Cas Number:
- 81-33-4
- IUPAC Name:
- isoquino[4',5',6':6,5,10]anthra[2,1,9-def]isoquinoline-1,3,8,10(2H,9H)-tetrone
- Details on test material:
- - Lot/batch No.: Partie 18
- Molecular formula: C24 H10 N2 O4
- Molecular weight (g/mol): 390.36
- Aggregate state: solid
- Water-solubility: <500 mg/L
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Concentration of sludge: 30 mg/I - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature:
- pH: 7.2 - 7.5
TEST SYSTEM
- Number of culture flasks/concentration: 7
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0 - 10
- Sampling time:
- 28 d
- Details on results:
- - Degradation degree of the test substance after 28 days (% BOD/ThOD) : 0-10
- Degradation degree of the test substance at the end of the test (% BOD/ThOD) : 0-10
BOD5 / COD results
- Results with reference substance:
- - Degradation degree of the reference substance after 14 days (% BOD/ThOD) : 70-80
Applicant's summary and conclusion
- Interpretation of results:
- other: poorly biodegradable
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