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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06/07/2011 to 08/27/2011
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Purchased from Shanghai Lanling Fishery
Batch no. BM20110518003
Held for 19 days in the laboratory to acclimatize to the test conditions, 12 hour photoperiod, 23 +/- 2°C, dissolved oxygen concentrations at least 80% of air saturation
No mortality recorded during the holding time
The fish were fed once daily, ceasing 24 hours prior to test initation
Length ranged from 2.6 to 2.9 cm
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
52 mg/L CaCO3
Test temperature:
23 +/- 2°C
pH:
6.0 to 8.5 initally, 7.32 to 8.15 after 24 hours of aeration
Dissolved oxygen:
6.79 to 8.47
Salinity:
not applicable
Nominal and measured concentrations:
The concentration was measured by HPLC. The minimum detectable concentration was 0.0007 mg/L. The concentration of the saturated solution was too low t detect by HPLC Method
Details on test conditions:
Three replicates of ten fish were used for the definitive test
Key result
Duration:
96 h
Remarks on result:
not determinable
Remarks:
saturated solution was below detection limits
Validity criteria fulfilled:
not specified
Conclusions:
No mortality was observed in the saturated solution, however the saturated solution was below the limit of detection (0.0007 mg/L).
Executive summary:

No mortality was observed in the saturated solution, however the saturated solution was below the limit of detection (0.0007 mg/L).

Description of key information

No toxicity observed in a saturated solution which was the highest concentration tested. This concentration was below the limit of detection of 0.0007 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
0.001 mg/L

Additional information