1,1'-[ethane-1,2-diylbis(thio)]bisbenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.46 (In Vitro Skin Irritation: Reconstructed Human Epidermis Model Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[ethane-1,2-diylbis(thio)]bisbenzene

EC Number:

210-723-5

EC Name:

1,1'-[ethane-1,2-diylbis(thio)]bisbenzene

Cas Number:

622-20-8

Molecular formula:

C14H14S2

IUPAC Name:

1,1'-(ethane-1,2-diyldisulfanediyl)dibenzene

Test material form:

solid

Remarks:

white solid

Details on test material:

The test substance is identified as 1,1'-[ethane-1,2-diylbis(thio)]bisbenzene. The purity is 100%. The physical state/appearance of material is white solid. The expiry date of material is 13 February 2019. The substance can be stored at room temperature in the dark conditions

Specific details on test material used for the study:

Purity is 100%

In vitro test system

Test system:

human skin model

Source species:

human

Cell type:

other: Adult human-derived epidermal keratinocytes seeded on a dermal substitute consisting of a collagen type I matrix coated with type IV collagen.

Cell source:

other: A highly differentiated and stratified epidermis model is obtained after a 13-Day culture period comprising of the main basal, supra basal, spinous and granular layers and a functional stratum corneum from an adult human.

Source strain:

not specified

Control samples:

yes, concurrent negative control

yes, concurrent positive control

Amount/concentration applied:

Approximately 10 mg (26.3 mg/cm2) of the test item was then applied to the epidermal surface. Purity of substance was 100%.

Duration of treatment / exposure:

Tissues were treated with the test item for an exposure period of 15 minutes

Duration of post-treatment incubation (if applicable):

42-Hour post-exposure incubation period

Number of replicates:

Triplicate tissues were treated with the test item.

Test system

Details on study design:

Pre-Test Procedure
To identify this possible interference, the test item is checked for the ability to directly reduce MTT.

Pre-incubation (Day 0: Tissue Arrival)
2 mL of maintenance medium, warmed to approximately 37 °C, was pipetted into the first column of 3 wells of a pre-labeled 12-well plate. Each epidermis unit was transferred into the maintenance medium filled wells (3 units per plate). A different 12-well plate was used for the test item and each control item. The tissues were incubated at 37 °C, 5% CO2 in air overnight.

Main test

Application of Test Item and Rinsing (Day 1)

Triplicate tissues were treated with the test item for an exposure period of 15 minutes.Approximately 10 mg (26.3 mg/cm2) of the test item was applied to the epidermal surface.Triplicate tissues treated with 10 μL of DPBS served as the negative controls and triplicate tissues treated with 10 μL of SDS 5% w/v served as the positive controls.At the end of the exposure period, each tissue was removed from the well using forceps and rinsed using a wash bottle containing DPBS with Ca++ and Mg++. Rinsing was achieved by filling and emptying each tissue insert for approximately 40 seconds using a constant soft stream of DPBS to gently remove any residual test item. The rinsed tissues were transferred to the second column of 3 wells containing 2 mL of maintenance medium in each well. The rinsed tissues were incubated at 37 °C, 5% CO2 in air for 42 hours.

MTT Loading/Formazan Extraction (Day 3)
2 mL of a 0.3 mg/mL MTT solution, freshly prepared in assay medium, was pipetted into the third column of 3 wells of the 12-well plates. The tissues were transferred to the MTT filled wells, being careful to remove any excess maintenance medium from the bottom of the tissue insert by blotting on absorbent paper. The tissues were incubated for 3 hours at 37 °C, 5% CO2 in air. At the end of the 3-Hour incubation period each tissue was placed onto absorbent paper to dry. A total biopsy of the epidermis was made using the EPISKINTM biopsy punch. The epidermis was carefully separated from the collagen matrix using forceps and both parts (epidermis and collagen matrix) placed into labeled 1.5 mL micro tubes containing 500 μL of acidified isopropanol, ensuring that both the epidermis and collagen matrix were fully immersed. Each tube was plugged to prevent evaporation and mixed thoroughly on a vortex mixer. The tubes were refrigerated at 1 to 10 °C until Day 6 of the experiment, allowing the extraction of formazan crystals out of the MTT-loaded tissues.

Absorbance/Optical Density Measurements (Day 6)
The optical density was measured at 570 nm. Data are presented in the form of percentage viability (MTT reduction in the test item treated tissues relative to negative control tissues).

Results and discussion

In vitro

Results

Irritation / corrosion parameter:

% tissue viability

Value:

105.7

Negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

no indication of irritation

Applicant's summary and conclusion

Interpretation of results:

other: No category. Not requiring classification to UN GHS or EU CLP

Conclusions:

The test item is not a skin irritant.

Executive summary:

The skin irritation potential of the 1,1'-[ethane-1,2-diylbis(thio )]bisbenzene was evaluated using the EPISKIN reconstructed human epidermis model after a treatment period of 15 minutes followed by a post-exposure incubation period of 42 hours.Triplicate tissues were treated with the test item for an exposure period of 15 minutes. At the end of the post-exposure incubation period tissues were taken for MTT-loading. After MTT-loading a total biopsy of each epidermis was made for extraction of formazan crystals. At the end of the formazan extraction period the optical density was measured at 570 nm. The percentage viability (MTT reduction in the test item treated tissues relative to negative control tissues) was calculated. The relative mean viability of the test item treated tissues was 105.7% after the 15-minute exposure period and 42 -hours post-exposure incubation period.The relative mean viability of negative control was 100% and for positive control was 6.8%.

The test substance is not an skin irritant.The test item is not requiring classification for skin irritancy under either UN GHS or EU CLP.