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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 May 2011 to 26 June 2011
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
none
Vehicle:
yes
Remarks:
10 mL acetone
Details on preparation and application of test substrate:
- Method of application to filter paper (if used):
- Method of mixing into soil (if used):
- Method of application to soil surface (if used): in solvent
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): aceton
- Concentration of vehicle in test medium (stock solution and final test solution): 10mL
- Evaporation of vehicle before use:yes
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
The earthworms were purchased from Shanghair, Minhang Earthworm Farm as adults, at least two months old with clitellum and with an indivdual weight of 0.3 to 0.6 g.
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20 +/- 2°C
pH:
6.0 +/- 0.5
Moisture:
35%
Details on test conditions:
TEST SYSTEM
- Test container (material, size):1L glassware
- Amount of soil or substrate:
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 2
- No. of replicates per control: 2
- No. of replicates per vehicle control: 2

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location:
- Pesticide use history at the collection site:
- Collection procedures:
- Sampling depth (cm):
- Soil texture (if natural soil)
- % sand:
- % silt:
- % clay:
- Soil taxonomic classification:
- Soil classification system:
- Composition (if artificial substrate): 10% sphagum peat, 20% kaolin clay and 70% industrial san
- Organic carbon (%):
- Maximum water holding capacity (in % dry weigth):
- CEC:
- Pretreatment of soil:
- Storage (condition, duration):
- Stability and homogeneity of test material in the medium:

OTHER TEST CONDITIONS
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Nominal and measured concentrations:
1000 mg/kg nominal
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
The LC50 for earthworms was reported as >1,000 kg/mg dry soil.
Executive summary:

An OECD 207 Earthworm acute toxicity study was conducted. A preliminary study indicated that the defintive study could be conducted as a Limit Test. The reported 14-d LC50 value was greater than the highest nominal test concentration of 1,000 mg/kg.

Description of key information

An OECD 207 acute earthworm toxicity test, conducted as a Limit Test report a 14 -day LC50 value of 1,000 mg/kg dry soil weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information