Cyclohexanethiol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1970

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a test protocol that is comparable to the appropriate OECD test guideline. It was not compliant with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexanethiol

EC Number:

216-378-7

EC Name:

Cyclohexanethiol

Cas Number:

1569-69-3

Molecular formula:

C6H12S

IUPAC Name:

cyclohexanethiol

Test material form:

other: liquid

Details on test material:

- Name of test material: Cyclohexyl Mercaptan
No other information reported

Test animals

Species:

rat

Strain:

other: Albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 100 to 118 g
- Fasting period before study: 16 to 24 hours before dosing
- Housing: Standard laboratory rat stock cages
- Diet: ad libitum(except before dosing, see above)
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 20% w/v

Doses:

0.900, 1.350, 2.025, 3.038 g/kg

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes

Statistics:

Acute oral mean lethal dose (LD50) was calculated using tables contructed by Weil (based on formulae presented by Thompson and Weil and involving moving averages and interpolation as developed by Thompson).

Thompson, William R and Weil, Carrol S.: On the Construction of Tables for Moving Average Interpolation. Biometrics, March 1952
Thompson, William R.: Use of Moving Averages and Interpolation to Estimate Median-Effective Dose. Bact. Rev., Nov. 1947

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1.22 other: g/kg

Based on:

test mat.

Mortality:

All animals at 3.038 or 2.025 g/kg died from 5.5 hours to 12 days after dose administration. One male and 1 female at 1.350 g/kg died 4 days after dosing.

There were no deaths in 0.900 g/kg animals.

Clinical signs:

other: Lethargy and/or generalised weakness and diarrhea were noted at all doses from immediately after dosing up to Day 7.

Gross pathology:

Hyperemic lungs, intestinal mucosa and pyloric region of stomach, together with prescence of blood in the gastro-intestinal tract, were noted in rats at all doses except 0.900 g/kg.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

In an acute oral toxicity study rats, following a protocol that is similar to the now-deleted OECD 401 test guideline, the LD50was calculated to be 1.22 g/kg +/- 0.12 g/kg.