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EC number: 229-066-0
CAS number: 6408-72-6
The ready biodegradability was
determined with non-adapted activated sludge in the Manometric
Respirometry Test for a period of 28 days. The study was conducted
according to OECD guideline 301 F. The test item concentration selected
as appropriate was 25 mg/L, corresponding to a ThOD of 50.8 mg 02/L
(58.3 mg 02/L with nitrification) per test vessel. The oxygen
was depleted by the respiration of bacteria and the degradation was
followed by measuring the oxygen concentration. The biodegradation rate
is therefore expressed as the percentage SOD (biological oxygen demand)
and was calculated for each study day. The mean oxygen depletion in the
inoculum control was 2.8 mg 02/L on day 28. In order to check
the activity of the test system sodium benzoate was used as functional
control. The pass level > 60 % was reached within 2 days. The
biodegradation reached a maximum of 98 % degradation on day 23. In the
toxicity control containing both test and reference item 55 %
degradation occurred within 14 days. After 28 days the biodegradation
came to 59 %. The degradation of the reference item was not inhibited by
the test item. The biodegradation remained in the range 0-8 % until test
end and the 60 % pass level was not reached within 28 days. The validity
criteria of the guideline are fulfilled.
Within 28 days, a degradation rate
of 5 % was determined. Solvent Violet 59 is classified as not readily
biodegradable in the 10-day-window and within 28 days. The reference
compound sodium benzoate showed 79 % degradation after 14 days.
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