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EC number: 229-066-0
CAS number: 6408-72-6
No toxic effects against fish were
observed at the limit of water solubility under exposure conditions.
method described in the Council Regulation (EC) No 440/2008, Method C.1
'Acute toxicity for Fish' (2008) which is in most parts equivalent to
the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute
Toxicity Test' (1992) assesses the acute toxic effects (mortality) of
various concentrations to a freshwater fish species. The purpose of this
method was to determine that concentration which causes a 50 % mortality
rate (= LC 50) or, if conducted as a limit test, to determine the acute
toxic effects at a maximum test concentration of 10.0 mg/L or at the
limit of water solubility. An algal growth inhibition test preceded the
fish test. It provided information about the concentration which was
used in the fish test. The following nominal concentrations of Solvent
Violet 59 were tested in the algal growth inhibition range-finding test:
1.0 and 100.0 mg/L. Because of the low solubility of lower than 1 mg/L
fish were exposed in the main test to a limit test concentration of
nominally 500 mg/L for a period of 96 hours. As no toxic effects against
fish were observed, no statistical analysis was required to determine
the LC 50. Additionally any abnormal behaviour or appearance of the fish
was reported every 24 hours.
96 hours of exposure a LC50 of > 500 mg/L was determined. No toxic
effects against fish were observed at the limit of water solubility
under exposure conditions. This toxicity study is classified acceptable
and satisfies the guideline requirement for the acute fish.
After 96 hours of exposure no effects against fish were observed up to a concentration of 500 mg/L.
"Should read as LC50 > 500 mg/L"
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