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EC number: 229-066-0
CAS number: 6408-72-6
Twenty-three fractions were
collected from column 1 and eleven fractions from column 2, which were
used for the determination of the water solubility. From each fraction
10 mL were collected.
The study was performed according
to guideline OECD 105 by the column elution method. For the support
material approximately 600 mg silica gel Chromosorb W/AW (0.15 - 0.18
mm; Merck) was transferred to a distilling flask. 21.6 mg of the test
item was weighed into a 100 mL measuring flask. Acetone was added to
dissolve the test substance and the flask was filled up to the mark with
acetone. 5 mL of this solution were diluted up to 100 mL with acetone. 5
mL of this solution was added to the support material and the solvent
was completely evaporated using a rotary evaporator. The support
material was soaked in demineralized water and the suspension was poured
into the water-filled micro column. After equilibration 10 mL fractions
from the micro column were collected and their concentration. was
determined by HPLC-MS analysis. The content of the Solvent violet 59 in
the test item was 99.2 %. The water solubility of Solvent violet 59 was
determined to be lower than 2.26 µg/L measured at 20 °C (pH-value
The water solubility of Solvent
violet 59 was determined to be lower than 2.26 µg/Lmeasured at 20 °C (pH-value
In a supporting study (Prokschy 2006) a water solubility of 4.9 µg/L was
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