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EC number: 297-628-2
CAS number: 93685-80-4
- Temperature in the exposure vessels during
96 hrs of exposure was 15.0 +/- 0.7 °C.
- The fish in the control series showed
normal swimming behaviour showing a cumulative immobilization/mortality
of 0% after 96 -hr exposure.
- Saturation of oxygen in the control series
and the highest concentration of the test did not fall below 85% during
- The fish in the sensitivity control series
exposed to 0.53 mg/L of 3.5 -dichlorophenol behaved lethagic but with
normal swimming behaviour and a cumulative immobilisation/mortality of
20% after 96 -hr exposure. A second sensitivity control series exosed to
0.67 mg/l of 3,5 -dichlorophenol behaved lethargic after 24 hr exposure
with a cumulative immobilization/mortality of 100% after 48 hr exposure.
An acute toxicity test was conducted on
juveniles of the marine fish Scophthalmus maximus (Turbot). The
test substance concentrations (nominal loading rates) ranged from 27 to
1028 mg/L. Test solutions were prepared as individual water accomodated
fractions (WAF). Control and dilution water were natural seawater of 32
-34 0/00 salinity filtered through a gravel filter
to 50 -70 µm. Test substance was mixed in dilution water for 20 hrs and
the mixture was allowed to settle for a minimum of 2.5 hr prior to
drawing off the aqueous solution for testing. Test vessels were 35 -L
white polyethylene vessels, each containing 10 L of test solution. In
the test, 5 concentrations were tested in a geometric series: 27, 66,
165, 410, and 1028 mg/L. Two replicates each containing 7 fishes
(biomass loading rate: 2.91 g/L) for the testing and 1 replicate
containing 10 fishes (biomass loading rate: 1.38 g/L) for the control
Dissolved oxygen was >60% saturation. The pH
was 7.80 - 8.03. The temperature was 15.0 +/- 0.7 °C.
Day 0 (initiation)
Day 4 (96 hrs)
Test organisms were obtained from a fish
farm (Stolt Sea Farms, Oye, Norway). At the test start, the organisms
were 40 - 60 mm long juveniles.
No deviations from the protocol were noted.
Analytical monitoring was not performed.
Therefore the results are based on the loading rate.
Nominal loading (mg/L)
Number of fish
7 x 2
(based upon nominal loading rate).
The water accommodated fraction of the
test substance produced a 96-hour LL50 value of >87,556 mg/L and LL0
value of 87,556 mg/L to the fish, Oncorhynchus mykiss. No mortality
was observed in either the control or treatment systems.
The fish were exposed to water accomodated
fractions (WAFs) and the results expressed as loading rates. The
reported LL50 values range from 0 >1028 mg/L to >70 g/L. No LC50
could be derived from acute short-term studies as they were only based
on loading. The risk assessment was performed using the Petrorisk model.
acute toxicity of Hydrocarbons, C14-C20, aliphatics, ≤2% aromatics was
tested on several different species of fish. Four study reports were
available and input as endpoint records. Three studies were
scientifically reliable according to Klimish (Klimish et al., 1997)
scoring (Klimish 1 or 2). The fourth one was scored 4 (unassignable)
despite of its scientific value because the test substance was not fully
to the low and variable solubility of the constituents of the substance
in water, most of the studies were conducted with water accommodated
fractions (WAFs). Marine fish Turbot (reported as Scophthalmus maximus,
presently Psetta maxima) was exposed to Hydrocarbons, C14-C18,
n-alkanes, isoalkanes, cyclics, <2% aromatics. WAFs and no toxic
effect/s (recorded as fish immobilization) was obtained with nominal
loadings up to 1028 mg/L (Altin, 2002). Studies conducted with
freshwater species (Calmels, 1994; Dufresne 2001a and b) lead to similar
results. Zebrafish (reported as Brachydanio rerio, presently Danio
rerio) exposure to the same substance as in the above turbot
experiment was performed using a mixture of acetone and emulsifier
Montanox: no mortality was recorded with nominal concentrations up to
250 mg/L. Dufresne (2001a) conducted a study where freshwater fish
fathead minnows Pimephales promelas were exposed to Hydrocarbons,
C12-C15,n-alkanes, <2% aromatics and Hydrocarbons, C14-C17,n-alkanes,
<2% aromatics as WAFs (1 part of test material + 10 parts of dilution
water - exposure to 12.5, 25, 50, 75 and 100% WAF) in a semi-static test
with renewal of the test medium every 48h. No significant mortality was
reported even with the highest concentration (100% WAF), corresponding
to 70 mg/L (nominal loading) for Hydrocarbons, C14-C17, n-alkanes, <2%
C14-C20, aliphatics, ≤2% aromatics show no acute toxicity towards fish:
the toxicity level was never reached in any study despite the high
loading level tested. Therefore, these substances are considered to
display no acute toxicity to fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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